Anti-GNA13 antibody [EPR5436]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (AB128900)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling GNA13 with Purified ab128900 at 1 : 1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (AB128900)

Unpurified ab128900, at a 1/250 dilution, staining GNA13 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (AB128900)

Unpurified ab128900, at a 1/250 dilution, staining GNA13 in paraffin embedded Human kidney tissue by Immunohistochemistry.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (AB128900)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling GNA13 with Purified ab128900 at 1 : 1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (AB128900)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling GNA13 with Purified ab128900 at 1 : 1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• WB

Lab

Western blot - Anti-GNA13 antibody [EPR5436] (AB128900)

Lanes 1 - 2 : Merged signal (red and green). Green - ab128900 observed at 45 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab128900 was shown to react with GNA13 in wild-type HeLa cells in Western blot with loss of signal observed in GNA13 knockout cell line ab264846 (GNA13 knockout cell lysate ab257451). Wild-type HeLa and GNA13 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab128900 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-GNA13 antibody [EPR5436] (ab128900) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

GNA13 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human GNA13 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-gna13-knockout-hela-cell-line-ab264846'>ab264846</a>)

Predicted band size: 44 kDa

Observed band size: 45 kDa

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• WB

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Western blot - Anti-GNA13 antibody [EPR5436] (AB128900)

All lanes:

Western blot - Anti-GNA13 antibody [EPR5436] (ab128900) at 1/1000 dilution

Lane 1:

Y79 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 44 kDa

false

• WB

Lab

Western blot - Anti-GNA13 antibody [EPR5436] (AB128900)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GNA13 antibody [EPR5436] (ab128900) at 1/5000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Mouse brain lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

• WB

Lab

Western blot - Anti-GNA13 antibody [EPR5436] (AB128900)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GNA13 antibody [EPR5436] (ab128900) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

Exposure time: 30s

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-GNA13 antibody [EPR5436] (AB128900)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.