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AB154157

Anti-GNA15 antibody

5

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal GNA15 antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GNA15 aa 100 to C-terminus.

View Alternative Names

GNA16, GNA15, Guanine nucleotide-binding protein subunit alpha-15, G alpha-15, G-protein subunit alpha-15, Epididymis tissue protein Li 17E, Guanine nucleotide-binding protein subunit alpha-16, G alpha-16, G-protein subunit alpha-16

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GNA15 antibody (AB154157)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GNA15 antibody (AB154157)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GNA15 with ab154157 at 1/500 dilution. The image in the lower panel was co-stained with Hoechst 33342.

Western blot - Anti-GNA15 antibody (AB154157)
  • WB

Unknown

Western blot - Anti-GNA15 antibody (AB154157)

10% SDS PAGE

All lanes:

Western blot - Anti-GNA15 antibody (ab154157) at 1/1000 dilution

All lanes:

THP1 whole cell lysate at 30 µg

Predicted band size: 44 kDa

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Western blot - Anti-GNA15 antibody (AB154157)
  • WB

Supplier Data

Western blot - Anti-GNA15 antibody (AB154157)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GNA15 antibody (ab154157) at 1/1000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

GNA16 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GNA15 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P30679

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GNA15 also known as G-protein subunit alpha-15 functions as part of the G protein complex which transduces signals from G protein-coupled receptors (GPCRs) to intracellular effectors. This protein weighs approximately 43 kDa. GNA15 gets expressed in various cell types with a notably higher expression in immune cells like lymphocytes and monocytes. Its role is to facilitate the transduction of external signals into a cellular response contributing to the regulation of numerous physiological processes.
Biological function summary

GNA15 participates in signal transduction by interacting with a range of signaling molecules. This protein serves as a mediator that couples activated GPCRs to downstream effectors influencing cellular responses. GNA15 does not operate alone; it forms a part of a heterotrimeric complex composed of alpha beta and gamma subunits enabling the integration of signals that modulate cellular activities like proliferation differentiation and immune response.

Pathways

GNA15 actively participates in intracellular signaling pathways that are critical for immune responses and cellular communication. The protein gets involved in the phospholipase C (PLC) pathway linking it with inositol trisphosphate (IP3) and diacylglycerol (DAG) production. Within this pathway it interacts with other proteins such as PLC-beta which play key roles in signal transduction. Furthermore GNA15 associates with the mitogen-activated protein kinase (MAPK) pathway impacting cell growth and survival.

GNA15 has connections to conditions involving immune function and inflammation. Aberrations in its signaling pathway have been implicated in immune disorders and inflammatory diseases such as rheumatoid arthritis. Additionally GNA15 links with other proteins like PLC-beta in the context of inflammatory signal transduction highlighting its importance in disease etiology and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
See full target information GNA15
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 13:6113-6124 PubMed38187070

2024

Platelet-related gene risk score: a predictor for pancreatic cancer microenvironmental signature, chemosensitivity and prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Si Hu,Ming Jiang,Ying-Jian Wang,Shou-Fang Xu,Fei-Yu Jiang,Ye-Tao Han,Zhi-Wei Liu,Hong Yu

PloS one 12:e0188366 PubMed29190729

2017

Increased MMAB level in mitochondria as a novel biomarker of hepatotoxicity induced by Efavirenz.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimi Tan,Xiaofang Jia,Fang Ma,Yanling Feng,Hongzhou Lu,Jun-O Jin,Dage Wu,Lin Yin,Li Liu,Lijun Zhang
View all publications
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