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AB220277

Anti-GNAI1 antibody

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(1 Publication)

Rabbit Polyclonal GNAI1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GNAI1 aa 100-150.

View Alternative Names

Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-GNAI1 antibody (AB220277)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GNAI1 antibody (AB220277)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cells labeling GNAI1 with ab220277 at 4 μg/ml (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GNAI1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P63096

Specificity

Similarity with other sequences from the G protein family is predicted but has not being tested empirically.

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Alternative Products

Primary Antibodies

AB202292

Alexa Fluor® 488 Anti-GNAI1 antibody [EPR9441(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GNAI1 antibody [EPR9441(B)] (AB202292)

  • 0
  • 0
Primary Antibodies

AB140125

Anti-GNAI1 antibody [EPR9441(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (AB140125)

  • 3
  • 2

Complementary Products

Primary Antibodies

AB157204

Anti-GNAI2 antibody [EPR9469]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI2 antibody [EPR9469] (AB157204)

  • 0
  • 0
Primary Antibodies

AB249914

Anti-GNAI3 antibody [EPR11900] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI3 antibody [EPR11900] - BSA and Azide free (AB249914)

  • 0
  • 0
Proteins & Peptides

AB113585

Recombinant Human GNAI1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GNAI1 protein (His tag N-Terminus) (AB113585)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GNAI1 also known as G protein subunit alpha i1 is part of the G-protein family that plays an important role in signal transduction. GNAI1 has a molecular mass of approximately 40 kDa. It is mainly expressed in neuronal tissues where it interacts with receptors to regulate intracellular signaling pathways. This protein operates by inhibiting adenylate cyclase activity which reduces the production of cyclic AMP (cAMP) in response to extracellular signals.
Biological function summary

GNAI1 acts by interfering with signal transmission across membranes. It is part of the heterotrimeric G-protein complex which is essential for many signaling processes. This complex responds to activation by G-protein-coupled receptors (GPCRs) and modulates various downstream effectors essentially affecting numerous physiological processes like neurotransmission and hormone signaling.

Pathways

GNAI1 plays a significant role in the cyclic AMP (cAMP) signaling pathway and the neuroactive ligand-receptor interaction pathway. In the cAMP pathway GNAI1 associates with receptors like dopamine and serotonin receptors helping to regulate neuronal response and neurotransmitter release. This activity involves interactions with related proteins such as GNB1 and GNG2 which form the beta and gamma subunits of the heterotrimeric G-protein complex.

GNAI1 relates to neuropathic conditions such as epilepsy and depression. Alterations in GNAI1 function can disrupt normal signaling contributing to these disorders. The protein interacts with other proteins in the signaling cascade such as RGS proteins which can affect its function in disease states. Researchers continue to explore how modifications or mutations in GNAI1 might offer potential therapeutic targets for these and similar disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 18434541, PubMed : 33762731, PubMed : 34239069, PubMed : 35610220, PubMed : 37935376, PubMed : 37935377, PubMed : 37963465, PubMed : 38552625, PubMed : 8774883, PubMed : 38918398, PubMed : 40080544). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 18434541, PubMed : 8774883). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 18434541, PubMed : 8774883). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 18434541, PubMed : 8774883). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 18434541, PubMed : 8774883). Signaling is mediated via effector proteins, such as adenylate cyclase : inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels (PubMed : 8119955). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed : 17635935). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed : 22327364).
See full target information GNAI1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical medicine 11: PubMed35160302

2022

Cyclin D1 Binding Protein 1 Responds to DNA Damage through the ATM-CHK2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Niwa,Kenya Kamimura,Kohei Ogawa,Chiyumi Oda,Yuto Tanaka,Ryoko Horigome,Masato Ohtsuka,Hiromi Miura,Koichi Fujisawa,Naoki Yamamoto,Taro Takami,Shujiro Okuda,Masayoshi Ko,Takashi Owaki,Atsushi Kimura,Osamu Shibata,Shinichi Morita,Norihiro Sakai,Hiroyuki Abe,Takeshi Yokoo,Akira Sakamaki,Hiroteru Kamimura,Shuji Terai
View all publications
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Product promise

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