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Rabbit Recombinant Monoclonal GNAI1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.


Images

Western blot - Anti-GNAI1 antibody [EPR9441(B)] (AB140125), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-GNAI1 antibody [EPR9441(B)] (AB140125), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (AB140125), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (AB140125), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Mouse
Predicted
Not recommended
Predicted
Predicted
Rat
Predicted
Not recommended
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/999 - 1/9999

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Product

Target data

Function

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (By similarity). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed:17635935). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed:22327364).

Alternative names

Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1

Recommended products

Rabbit Recombinant Monoclonal GNAI1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

Alternative names

Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR9441(B)

Purification technique

Affinity purification Protein A

Specificity

The immunogenic sequence of ab140125 has 92.9% homology with GNAI3 and 64% (4 continuous residues) homology with GNAI2 which might cause cross reactivity with GNAI3 and GNAI2.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein GNAI1 also known as G protein subunit alpha i1 is part of the G-protein family that plays an important role in signal transduction. GNAI1 has a molecular mass of approximately 40 kDa. It is mainly expressed in neuronal tissues where it interacts with receptors to regulate intracellular signaling pathways. This protein operates by inhibiting adenylate cyclase activity which reduces the production of cyclic AMP (cAMP) in response to extracellular signals.

Biological function summary

GNAI1 acts by interfering with signal transmission across membranes. It is part of the heterotrimeric G-protein complex which is essential for many signaling processes. This complex responds to activation by G-protein-coupled receptors (GPCRs) and modulates various downstream effectors essentially affecting numerous physiological processes like neurotransmission and hormone signaling.

Pathways

GNAI1 plays a significant role in the cyclic AMP (cAMP) signaling pathway and the neuroactive ligand-receptor interaction pathway. In the cAMP pathway GNAI1 associates with receptors like dopamine and serotonin receptors helping to regulate neuronal response and neurotransmitter release. This activity involves interactions with related proteins such as GNB1 and GNG2 which form the beta and gamma subunits of the heterotrimeric G-protein complex.

Associated diseases and disorders

GNAI1 relates to neuropathic conditions such as epilepsy and depression. Alterations in GNAI1 function can disrupt normal signaling contributing to these disorders. The protein interacts with other proteins in the signaling cascade such as RGS proteins which can affect its function in disease states. Researchers continue to explore how modifications or mutations in GNAI1 might offer potential therapeutic targets for these and similar disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-GNAI1 antibody [EPR9441(B)] (ab140125), expandable thumbnail

    Western blot - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)

    All lanes: Western blot - Anti-GNAI1 antibody [EPR9441(B)] (ab140125) at 1/1000 dilution

    Lane 1: SH-SY5Y cell lysate at 10 µg

    Lane 2: U-87 MG cell lysate at 10 µg

    Lane 3: Human glioma tissue lysate at 10 µg

    Lane 4: Human fetal brain tissue lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 40 kDa

  • Flow Cytometry (Intracellular) - Anti-GNAI1 antibody [EPR9441(B)] (ab140125), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)

    Overlay histogram showing SHSY-5Y cells stained with ab140125 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140125, 1/9999 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (ab140125), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling GNAI1 with ab140125 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (ab140125), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)

    Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labelling GNAI1 with ab140125 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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