Anti-GNAI1 antibody [EPR9441(B)] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] - BSA and Azide free (AB248888)

This data was developed using ab140125, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labelling GNAI1 with ab140125 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] - BSA and Azide free (AB248888)

This data was developed using ab140125, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling GNAI1 with ab140125 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GNAI1 antibody [EPR9441(B)] - BSA and Azide free (AB248888)

This data was developed using ab140125, the same antibody clone in a different buffer formulation.

Overlay histogram showing SHSY-5Y cells stained with ab140125 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140125, 1/9999 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

Unknown

Western blot - Anti-GNAI1 antibody [EPR9441(B)] - BSA and Azide free (AB248888)

This data was developed using ab140125, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GNAI1 antibody [EPR9441(B)] (<a href='/en-us/products/primary-antibodies/gnai1-antibody-epr9441b-ab140125'>ab140125</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

U-87 MG cell lysate at 10 µg

Lane 3:

Human glioma tissue lysate at 10 µg

Lane 4:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 40 kDa

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