AB3522

Anti-GNAI1 + GNAI2 antibody

BOND RX™ Validated
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(5 Publications)

Rabbit Polyclonal GNAI1 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human GNAI1 aa 300-400.

View Alternative Names

Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1, GNAI2B, GNAI2, Guanine nucleotide-binding protein G(i) subunit alpha-2, Adenylate cyclase-inhibiting G alpha protein

2 Images

Supplier Data

Immunocytochemistry - Anti-GNAI1 + GNAI2 antibody (AB3522)

SH-SY5Y cells stained for GNAI1 (green) using ab3522 at 2 μg/mL in immunocytochemical analysis. The nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI, F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel e shows the no primary antibody control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 + GNAI2 antibody (AB3522)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 + GNAI2 antibody (AB3522)

IHC image of ab3522 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3522, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IHC-P

applications

Immunogen

Synthetic Peptide within Human GNAI1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

P63096

Specificity

Similarity with other sequences from the G protein family is predicted but has not being tested empirically.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "<p></p>" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Starfish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNAI1 and GNAI2 also known as Gαi1 and Gαi2 are G protein subunits that belong to the Gi/Go family of proteins. These proteins have a molecular mass of approximately 40 kDa each. Mechanically they inhibit the production of cyclic AMP (cAMP) by binding to adenylate cyclase. GNAI1 and GNAI2 are expressed throughout various tissues including the brain heart and immune cells where they play a role in signal transduction processes.

Biological function summary

These proteins modulate intracellular signaling by interacting with seven-transmembrane domain receptors. GNAI1 and GNAI2 participate in heterotrimeric G protein complexes acting as transducers in signal transduction pathways. They are key players in mediating receptor responses to hormones and neurotransmitters influencing cellular responses such as growth migration and secretion.

Pathways

GNAI1 and GNAI2 significantly influence the cAMP-dependent signaling pathway and the PI3K-Akt signaling pathway. These pathways are critical for cellular functions like cell proliferation and survival. GNAI proteins including GNAI1 and GNAI2 often interact with other proteins like adenylate cyclase and phosphoinositide 3-kinases (PI3Ks) to modulate these pathways and ensure proper cellular responses.

GNAI1 and GNAI2 have been linked to specific conditions such as hypertension and some immune disorders. Hypertension involves complex interactions where GNAI1 and GNAI2 modulate vascular tone through cAMP signaling. Furthermore these proteins connect to signaling components like adrenergic receptors and other G proteins in disease contexts impacting disease pathogenesis and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 18434541, PubMed : 33762731, PubMed : 34239069, PubMed : 35610220, PubMed : 37935376, PubMed : 37935377, PubMed : 37963465, PubMed : 38552625, PubMed : 8774883, PubMed : 38918398, PubMed : 40080544). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 18434541, PubMed : 8774883). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 18434541, PubMed : 8774883). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 18434541, PubMed : 8774883). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 18434541, PubMed : 8774883). Signaling is mediated via effector proteins, such as adenylate cyclase : inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels (PubMed : 8119955). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed : 17635935). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed : 22327364).

See full target information GNAI1

Additional targets

GNAI2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36010658

2022

Expression of Functional Cannabinoid Type-1 (CB) Receptor in Mitochondria of White Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio C Pagano Zottola,Ilenia Severi,Astrid Cannich,Philippe Ciofi,Daniela Cota,Giovanni Marsicano,Antonio Giordano,Luigi Bellocchio

Biochemical pharmacology 154:452-463 PubMed29883706

2018

Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of δ-opioid receptor amount and function.

Applications

Unspecified application

Species

Unspecified reactive species

Miroslava Vosahlikova,Hana Ujcikova,Martina Hlouskova,Stanislav Musil,Lenka Roubalova,Martin Alda,Petr Svoboda

Journal of neuroimmunology 321:12-23 PubMed29957382

2018

Up-regulation of μ-, δ- and κ-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Cechova,Martina Hlouskova,Eliska Javorkova,Lenka Roubalova,Hana Ujcikova,Vladimir Holan,Petr Svoboda

Nature communications 7:10583 PubMed26882873

2016

Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour.

Applications

Species

Unspecified reactive species

Masao Doi,Iori Murai,Sumihiro Kunisue,Genzui Setsu,Naohiro Uchio,Rina Tanaka,Sakurako Kobayashi,Hiroyuki Shimatani,Hida Hayashi,Hsu-Wen Chao,Yuuki Nakagawa,Yukari Takahashi,Yunhong Hotta,Jun-ichirou Yasunaga,Masao Matsuoka,Michael H Hastings,Hiroshi Kiyonari,Hitoshi Okamura

British journal of pharmacology 158:300-13 PubMed19719783

2009

Cross-regulation between beta 1- and beta 3-adrenoceptors following chronic beta-adrenergic stimulation in neonatal rat cardiomyocytes.

Applications

Species

Rat

Christoph Ufer,Renée Germack

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GNAI1 + GNAI2 antibody

Immunocytochemistry - Anti-GNAI1 + GNAI2 antibody (AB3522)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 + GNAI2 antibody (AB3522)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (5)

Product promise