AB317135

Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093]

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal GNAI1 antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Human, Mouse, Rat, Zebrafish samples.

View Alternative Names

Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1, GNAI2B, GNAI2, Guanine nucleotide-binding protein G(i) subunit alpha-2, Adenylate cyclase-inhibiting G alpha protein, Guanine nucleotide-binding protein G(i) subunit alpha-3, G(i) alpha-3, GNAI3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (AB317135)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (AB317135)

ab317135 detects GNAI1 + GNAI2 + GNAI3 protein at cytoplasm by immunofluorescent analysis. Sample : HeLa cells were fixed in ice-cold MeOH for 5 min. Green : GNAI1 + GNAI2 + GNAI3 stained by ab317135 diluted at 1 : 500. Red : alpha Tubulin a cytoskeleton marker stained by alpha Tubulin antibody [GT114] diluted at 1 : 1000. Blue : Fluoroshield with DAPI. Scale bar= 10um.

Supplier Data

Western blot - Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (AB317135)

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317135 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (ab317135) at 1/1000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (AB317135)

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317135 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (ab317135) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2093

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat, Zebrafish

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNAI3 GNAI1 and GNAI2 also known as Gi alpha subunits belong to the G protein family which are involved in intracellular signaling. These proteins have molecular masses of approximately 40 kDa. They are expressed in a variety of tissues including the brain heart and immune cells. Each subunit interacts with the beta and gamma subunits of G proteins to form a complex which plays a role in signal transduction by inhibiting adenylate cyclase activity leading to decreased levels of cAMP.

Biological function summary

Gi alpha subunits regulate various physiological processes such as neurotransmission and immune response. They are integral parts of receptor-mediated signaling pathways binding to G-protein-coupled receptors (GPCRs) on cell surfaces. This interaction triggers changes in cAMP levels which in turn affects cellular responses to external stimuli. Gi alpha proteins serve as molecular switches turning on or off specific signaling pathways.

Pathways

Gi alpha proteins are involved in the regulation of the cAMP signaling and calcium signaling pathways. They interact with proteins such as beta-adrenergic receptors which modulate heart rate and force of contraction. GNAI3 GNAI1 and GNAI2 relay signals from various GPCRs playing key roles in translating extracellular signals into cellular actions. Such pathways are essential for maintaining homeostasis and responding to environmental changes.

Aberrations in Gi alpha protein functions associate with conditions such as pertussis and cardiovascular diseases. Pertussis toxin targets these proteins disrupting their normal function and affecting immune response. Additionally abnormal signaling through Gi alpha-related pathways is linked to hypertension as it involves other proteins in the renin-angiotensin system. Understanding the role of these subunits aids in the development of therapeutic interventions targeting GPCR-related pathologies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 18434541, PubMed : 33762731, PubMed : 34239069, PubMed : 35610220, PubMed : 37935376, PubMed : 37935377, PubMed : 37963465, PubMed : 38552625, PubMed : 8774883, PubMed : 38918398, PubMed : 40080544). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 18434541, PubMed : 8774883). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 18434541, PubMed : 8774883). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 18434541, PubMed : 8774883). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 18434541, PubMed : 8774883). Signaling is mediated via effector proteins, such as adenylate cyclase : inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels (PubMed : 8119955). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed : 17635935). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed : 22327364).

See full target information GNAI1

Additional targets

GNAI2,Guanine nucleotide-binding protein G(i) subunit alpha-3

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093]

Immunocytochemistry/ Immunofluorescence - Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (AB317135)

Western blot - Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (AB317135)

Western blot - Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (AB317135)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise