Skip to main content

Rabbit Polyclonal GNAI3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(i) subunit alpha-3 aa 50 to C-terminus.


Images

Western blot - Anti-GNAI3 antibody (AB154024), expandable thumbnail
  • Western blot - Anti-GNAI3 antibody (AB154024), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI3 antibody (AB154024), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(i) subunit alpha-3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P08754

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Predicted
Predicted
Chicken
Predicted
Predicted
Cow
Predicted
Predicted
Pig
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Xenopus tropicalis
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Chicken, Cow, Pig, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/3000

Notes

-

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Rat, Chicken, Cow, Pig, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:18434541, PubMed:19478087, PubMed:8774883). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).

Alternative names

Recommended products

Rabbit Polyclonal GNAI3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(i) subunit alpha-3 aa 50 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(i) subunit alpha-3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P08754
Purification technique

Affinity purification Immunogen

Specificity

Immunogen shares high homology with human GNAI1 and GNAI2, and with other proteins of the Galpha family and therefore cross-reactivity may occur.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GNAI3 also known as the guanine nucleotide-binding protein G(i) alpha-3 subunit is part of the heterotrimeric G protein family. Its molecular weight is approximately 41 kDa. This protein plays a role in transmitting signals from extracellular stimuli through cell-surface receptors to the intracellular signaling pathways. GNAI3 is expressed in various tissues including the brain and heart where it facilitates important cellular responses by interacting with other proteins in signaling pathways.

Biological function summary

GNAI3 contributes to inhibiting adenylate cyclase activity reducing cyclic AMP levels and therefore modulating downstream signaling events. It forms part of the heterotrimeric guanine nucleotide-binding protein complex which consists of alpha beta and gamma subunits. By interacting with this complex GNAI3 regulates processes like cell division immune response and sensory perception demonstrating its diverse functional roles within the cell.

Pathways

GNAI3 is involved in the G protein-coupled receptor (GPCR) signaling pathway which is essential for transmitting numerous extracellular signals to the inside of the cell. GNAI3 modulates signals from neurotransmitters and hormones that activate this pathway. Additionally GNAI3 interacts with other proteins such as beta-arrestins which are important for receptor desensitization and signal termination within the GPCR signaling system.

Associated diseases and disorders

Researchers have linked GNAI3 disruptions to auriculocondylar syndrome and certain forms of cancer. In auriculocondylar syndrome mutations in GNAI3 contribute to craniofacial abnormalities. Additionally alterations in its signaling pathways might contribute to tumor progression by failing to regulate cell proliferation properly. GNAI3 interacts with proteins including RGS proteins which help in temporally regulating signal transduction showing its significance in health and disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-GNAI3 antibody (ab154024), expandable thumbnail

    Western blot - Anti-GNAI3 antibody (ab154024)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-GNAI3 antibody (ab154024) at 1/1000 dilution

    Lane 1: A431 whole cell lysate at 30 µg

    Lane 2: MOLT4 whole cell lysate at 30 µg

    Lane 3: Raji whole cell lysate at 30 µg

    Predicted band size: 41 kDa

  • Western blot - Anti-GNAI3 antibody (ab154024), expandable thumbnail

    Western blot - Anti-GNAI3 antibody (ab154024)

    10% SDS PAGE

    All lanes: Western blot - Anti-GNAI3 antibody (ab154024) at 1/1000 dilution

    All lanes: Mouse brain whole cell lysate at 50 µg

    Predicted band size: 41 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI3 antibody (ab154024), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI3 antibody (ab154024)

    Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling GNAI3 with ab154024 at 1/500 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com