Rabbit Polyclonal GNAI3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(i) subunit alpha-3 aa 50 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
Rat | Predicted | Predicted |
Chicken | Predicted | Predicted |
Cow | Predicted | Predicted |
Pig | Predicted | Predicted |
Xenopus laevis | Predicted | Predicted |
Xenopus tropicalis | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Pig, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Pig, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Select an associated product type
Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:18434541, PubMed:19478087, PubMed:8774883). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).
Guanine nucleotide-binding protein G(i) subunit alpha-3, G(i) alpha-3, GNAI3
Rabbit Polyclonal GNAI3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(i) subunit alpha-3 aa 50 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Immunogen shares high homology with human GNAI1 and GNAI2, and with other proteins of the Galpha family and therefore cross-reactivity may occur.
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
GNAI3 also known as the guanine nucleotide-binding protein G(i) alpha-3 subunit is part of the heterotrimeric G protein family. Its molecular weight is approximately 41 kDa. This protein plays a role in transmitting signals from extracellular stimuli through cell-surface receptors to the intracellular signaling pathways. GNAI3 is expressed in various tissues including the brain and heart where it facilitates important cellular responses by interacting with other proteins in signaling pathways.
GNAI3 contributes to inhibiting adenylate cyclase activity reducing cyclic AMP levels and therefore modulating downstream signaling events. It forms part of the heterotrimeric guanine nucleotide-binding protein complex which consists of alpha beta and gamma subunits. By interacting with this complex GNAI3 regulates processes like cell division immune response and sensory perception demonstrating its diverse functional roles within the cell.
GNAI3 is involved in the G protein-coupled receptor (GPCR) signaling pathway which is essential for transmitting numerous extracellular signals to the inside of the cell. GNAI3 modulates signals from neurotransmitters and hormones that activate this pathway. Additionally GNAI3 interacts with other proteins such as beta-arrestins which are important for receptor desensitization and signal termination within the GPCR signaling system.
Researchers have linked GNAI3 disruptions to auriculocondylar syndrome and certain forms of cancer. In auriculocondylar syndrome mutations in GNAI3 contribute to craniofacial abnormalities. Additionally alterations in its signaling pathways might contribute to tumor progression by failing to regulate cell proliferation properly. GNAI3 interacts with proteins including RGS proteins which help in temporally regulating signal transduction showing its significance in health and disease contexts.
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7.5% SDS PAGE
All lanes: Western blot - Anti-GNAI3 antibody (ab154024) at 1/1000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: MOLT4 whole cell lysate at 30 µg
Lane 3: Raji whole cell lysate at 30 µg
Predicted band size: 41 kDa
10% SDS PAGE
All lanes: Western blot - Anti-GNAI3 antibody (ab154024) at 1/1000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 41 kDa
Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling GNAI3 with ab154024 at 1/500 dilution.
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