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AB154024

Anti-GNAI3 antibody

4

(1 Review)

|

(4 Publications)

Rabbit Polyclonal GNAI3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(i) subunit alpha-3 aa 50 to C-terminus.

View Alternative Names

Guanine nucleotide-binding protein G(i) subunit alpha-3, G(i) alpha-3, GNAI3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI3 antibody (AB154024)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI3 antibody (AB154024)

Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling GNAI3 with ab154024 at 1/500 dilution.

Western blot - Anti-GNAI3 antibody (AB154024)
  • WB

Unknown

Western blot - Anti-GNAI3 antibody (AB154024)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GNAI3 antibody (ab154024) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

MOLT4 whole cell lysate at 30 µg

Lane 3:

Raji whole cell lysate at 30 µg

Predicted band size: 41 kDa

false

Western blot - Anti-GNAI3 antibody (AB154024)
  • WB

Unknown

Western blot - Anti-GNAI3 antibody (AB154024)

10% SDS PAGE

All lanes:

Western blot - Anti-GNAI3 antibody (ab154024) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(i) subunit alpha-3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P08754

Specificity

Immunogen shares high homology with human GNAI1 and GNAI2, and with other proteins of the Galpha family and therefore cross-reactivity may occur.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNAI3 also known as the guanine nucleotide-binding protein G(i) alpha-3 subunit is part of the heterotrimeric G protein family. Its molecular weight is approximately 41 kDa. This protein plays a role in transmitting signals from extracellular stimuli through cell-surface receptors to the intracellular signaling pathways. GNAI3 is expressed in various tissues including the brain and heart where it facilitates important cellular responses by interacting with other proteins in signaling pathways.
Biological function summary

GNAI3 contributes to inhibiting adenylate cyclase activity reducing cyclic AMP levels and therefore modulating downstream signaling events. It forms part of the heterotrimeric guanine nucleotide-binding protein complex which consists of alpha beta and gamma subunits. By interacting with this complex GNAI3 regulates processes like cell division immune response and sensory perception demonstrating its diverse functional roles within the cell.

Pathways

GNAI3 is involved in the G protein-coupled receptor (GPCR) signaling pathway which is essential for transmitting numerous extracellular signals to the inside of the cell. GNAI3 modulates signals from neurotransmitters and hormones that activate this pathway. Additionally GNAI3 interacts with other proteins such as beta-arrestins which are important for receptor desensitization and signal termination within the GPCR signaling system.

Researchers have linked GNAI3 disruptions to auriculocondylar syndrome and certain forms of cancer. In auriculocondylar syndrome mutations in GNAI3 contribute to craniofacial abnormalities. Additionally alterations in its signaling pathways might contribute to tumor progression by failing to regulate cell proliferation properly. GNAI3 interacts with proteins including RGS proteins which help in temporally regulating signal transduction showing its significance in health and disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 18434541, PubMed : 19478087, PubMed : 8774883). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed : 19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed : 2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed : 17635935).
See full target information Guanine nucleotide-binding protein G(i) subunit alpha-3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:699 PubMed39349432

2024

Identification of Gαi3 as a promising molecular oncotarget of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Zhuo Jiang,Yin-Biao Qiao,Xiao-Ren Zhu,Qian-Hui Gu,Jing-Jing Lu,Zhen-Yu Ye,Lu Xu,Yuan-Yuan Liu

International journal of biological sciences 17:4238-4253 PubMed34803495

2021

Mir24-2-5p suppresses the osteogenic differentiation with Gnai3 inhibition presenting a direct target via inactivating JNK-p38 MAPK signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Meng,Lichan Yuan,Jieli Ni,Mengru Fang,Shuyu Guo,Huayang Cai,Jinwei Qin,Qi Cai,Mengnan Zhang,Fang Hu,Junqing Ma,Yang Zhang

Autophagy 18:357-374 PubMed34190023

2021

CCDC88A/GIV promotes HBV replication and progeny secretion via enhancing endosomal trafficking and blocking autophagic degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyu Wang,Zhiqiang Wei,Tingyu Lan,Yulin He,Bin Cheng,Ruimin Li,Hongxia Chen,Fahong Li,Guohua Liu,Bin Jiang,Yong Lin,Mengji Lu,Zhongji Meng

International journal of molecular medicine 43:382-392 PubMed30431055

2018

Guanine and nucleotide binding protein 3 promotes odonto/osteogenic differentiation of apical papilla stem cells via JNK and ERK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhang,Lichan Yuan,Li Meng,Mengru Fang,Shuyu Guo,Dongyue Wang,Junqing Ma,Lin Wang
View all publications
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