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AB74049

Anti-GNAL antibody

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(6 Publications)

Rabbit Polyclonal GNAL antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Guanine nucleotide-binding protein G(olf) subunit alpha aa 50-100.

View Alternative Names

Guanine nucleotide-binding protein G(olf) subunit alpha, GNAL

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAL antibody (AB74049)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAL antibody (AB74049)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab74049, in the absence (left) or presence (right) of the immunizing peptide.

Western blot - Anti-GNAL antibody (AB74049)
  • WB

Unknown

Western blot - Anti-GNAL antibody (AB74049)

All lanes:

Western blot - Anti-GNAL antibody (ab74049) at 1/500 dilution

Lane 1:

extracts from LOVO cells at 30 µg

Lane 2:

extracts from LOVO cells at 30 µg with immunizing peptide

Predicted band size: 44 kDa

Observed band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Guanine nucleotide-binding protein G(olf) subunit alpha aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P38405

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GNAL gene encodes a protein also known as guanine nucleotide-binding protein G(olf) subunit alpha. This protein with a mass of approximately 45 kDa is part of the G-protein family that couples with G-protein-coupled receptors (GPCRs). GNAL is mainly expressed in the brain particularly in the olfactory system and basal ganglia. It plays a role in signal transduction processes by modulating signaling pathways initiated by neurotransmitter receptors.
Biological function summary

Proteins encoded by GNAL participate in signal transduction pathways. These pathways often engage G-protein complexes where GNAL functions as the alpha subunit. It initiates the activation of intracellular signaling cascades upon interaction with activated receptors. GNAL contributes to neurotransmitter signaling which has implications in sensory perception a function dependent on the proper relay of chemical signals within the nervous system.

Pathways

GNAL focuses on neurotransmission and sensory processing. It significantly affects the dopamine signaling pathway important for motor control and reward-motivated behaviors. In this pathway GNAL interacts with dopamine receptors to mediate signal transduction working alongside other proteins such as DRD1. Additionally GNAL is involved in the olfactory transduction pathway important for detecting and processing odors emphasizing its specific expression in the olfactory bulb.

Defects or mutations in GNAL correlate with certain neurological disorders. They are linked to Dystonia where abnormal muscle contractions lead to twisting and repetitive movements. This connection occurs through altered GNAL-mediated dopamine signaling affecting DRD1 functions. Furthermore GNAL variants associate with schizophrenia owing to disruptions in neurotransmission pathways. Understanding the role of GNAL offers insights into its involvement in diseases related to dopamine pathway dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding protein (G protein) involved as transducer in olfactory signal transduction controlled by G protein-coupled receptors (GPCRs) (By similarity). Contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (By similarity). Signaling by an activated GPCR promotes GDP release and GTP binding (By similarity). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (By similarity). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (By similarity). GNAL/G(olf) alpha specifically mediates olfactory signal transduction within the olfactory neuroepithelium and the basal ganglia following GPCRs activation (By similarity). Acts by promoting the specific activation of adenylyl cyclase ADCY3, resulting in increased levels of the signaling molecule cAMP (By similarity).
See full target information Guanine nucleotide-binding protein G(olf) subunit alpha
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 26:1058-1068 PubMed39582799

2024

Orexin-A increases the differentiation of human olfactory sensory neurons through orexin receptor type 1.

Applications

Unspecified application

Species

Unspecified reactive species

Yin-Tzu Chen,Tai-Horng Young,Yu-Hsin Wang,Chih-Hsuan Huang,Yu-Yun Gao,Tsung-Wei Huang

Rhinology 56:336-342 PubMed30052693

2018

Chitosan films promote formation of olfactory neurospheres and differentiation of olfactory receptor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

S T Li,T H Young,C T Wang,T W Huang

Journal of proteome research 17:858-869 PubMed29215295

2017

Quantitative Proteomic Study Reveals Up-Regulation of cAMP Signaling Pathway-Related Proteins in Mild Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hai Song,Shanhua Fang,Jing Gao,Jiaxong Wang,Zhenzhen Cao,Zeyun Guo,Qiongping Huang,Yongqang Qu,Hu Zhou,Jianyun Yu

The FEBS journal 283:1748-66 PubMed26934374

2016

'Smelling' the cerebrospinal fluid: olfactory signaling molecules are expressed in and mediate chemosensory signaling from the choroid plexus.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Gonçalves,Peter C Hubbard,Joana Tomás,Telma Quintela,Gabriela Tavares,Sandra Caria,Daniela Barreiros,Cecília R A Santos

PloS one 10:e0123104 PubMed25860259

2015

Dopamine receptor and Gα(olf) expression in DYT1 dystonia mouse models during postnatal development.

Applications

WB

Species

Unspecified reactive species

Lin Zhang,Deirdre M McCarthy,Nutan Sharma,Pradeep G Bhide

Developmental cell 17:649-61 PubMed19922870

2009

MOR23 promotes muscle regeneration and regulates cell adhesion and migration.

Applications

WB

Species

Mouse

Christine A Griffin,Kimberly A Kafadar,Grace K Pavlath
View all publications
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