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AB117273

Anti-GNAO1 antibody

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(1 Publication)

Rabbit Polyclonal GNAO1 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Gnao1 aa 300-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Gna0, Gnao, Gnao1, Guanine nucleotide-binding protein G(o) subunit alpha

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody (AB117273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody (AB117273)

ab117273, at 10 μg/ml, staining GNAO1 in Formalin-fixed, Paraffin-embedded Mouse Brain cerebellum tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody (AB117273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody (AB117273)

ab117273, at 10 μg/ml, staining GNAO1 in Formalin-fixed, Paraffin-embedded Mouse Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Gnao1 aa 300-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P18872

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Precipitation Ammonium Sulphate
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNAO1 also known as Guanine nucleotide-binding protein G(o) subunit alpha acts mechanically as part of the signaling processes within cells. It is a G protein alpha subunit that weighs around 41 kDa. The protein is widely expressed in neurons particularly affecting neurotransmitter signal transduction in brain regions like the cortex and hippocampus. GNAO1 is a part of the heterotrimeric G protein family that transduces signals from receptors on the cell surface to internal effectors playing a central role in signal processing.
Biological function summary

GNAO1 regulates the activity of various downstream effectors contributing to multiple cellular responses. It partners with beta and gamma subunits in forming a heterotrimeric G protein complex. The GNAO1's impact includes modulating neurotransmitter release influencing neuronal excitability and controlling other cellular processes like gene transcription. This protein's involvement in these processes highlights its necessity for normal neurological function and synaptic signaling.

Pathways

GNAO1 engages in critical molecular pathways such as the G protein-coupled receptor (GPCR) signaling pathway and the MAPK signaling pathway. It acts in concert with proteins like GNAI1 and GNB1 within these pathways contributing to a variety of cellular responses by transmitting signals received from extracellular ligands to initiate intracellular actions. GNAO1's activity in these pathways ensures proper signaling cascade required for normal cellular responses.

Mutations or dysfunctions of GNAO1 can lead to neurological conditions such as epileptic encephalopathy and movement disorders like dystonia. It relates closely to disorders through its interaction with proteins like GNB1 which also influence similar neurological diseases. These associations underline GNAO1's significance in maintaining neurological health shedding light on potential therapeutic targets for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 10926822). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 10926822). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 10926822). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 10926822). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 10926822). Signaling is mediated via effector proteins, such as adenylate cyclase (PubMed : 10926822). Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed : 10926822).
See full target information Gnao1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 19: PubMed30103548

2018

Prostanoid EP₂ Receptors Are Up-Regulated in Human Pulmonary Arterial Hypertension: A Key Anti-Proliferative Target for Treprostinil in Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jigisha A Patel,Lei Shen,Susan M Hall,Chabha Benyahia,Xavier Norel,Robin J McAnulty,Shahin Moledina,Adam M Silverstein,Brendan J Whittle,Lucie H Clapp
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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