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AB154001

Anti-GNAO1 antibody

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(5 Publications)

Rabbit Polyclonal GNAO1 antibody. Suitable for WB, IP, ICC/IF, IHC-P and reacts with Mouse, Human, Rat, Zebrafish samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GNAO1 aa 100-350.

View Alternative Names

Guanine nucleotide-binding protein G(o) subunit alpha, GNAO1

6 Images
Immunoprecipitation - Anti-GNAO1 antibody (AB154001)
  • IP

Supplier Data

Immunoprecipitation - Anti-GNAO1 antibody (AB154001)

Immunoprecipitation of GNAO1 protein from HeLa whole cell extracts using 5 µg of ab154001. Western blot analysis was performed using ab154001.

All lanes:

Immunoprecipitation - Anti-GNAO1 antibody (ab154001) at 5 µg

Lane 1:

HeLa whole cell extracts (Input)

Lane 2:

HeLa whole cell extracts with IgG control

Lane 3:

HeLa whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 40 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-GNAO1 antibody (AB154001)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GNAO1 antibody (AB154001)

Immunofluorescence analysis of DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling GNAO1 protein using ab154001 at a 1/500 dilution (Green). beta Tubulin 3/ Tuj1, stained by using an anti-beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

Western blot - Anti-GNAO1 antibody (AB154001)
  • WB

Supplier Data

Western blot - Anti-GNAO1 antibody (AB154001)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GNAO1 antibody (ab154001) at 1/1000 dilution

Lane 1:

U87-MG whole cell lysates at 30 µg

Lane 2:

SK-N-SH whole cell lysates at 30 µg

Lane 3:

IMR32 whole cell lysates at 30 µg

Lane 4:

SK-N-AS whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40 kDa

false

Western blot - Anti-GNAO1 antibody (AB154001)
  • WB

Supplier Data

Western blot - Anti-GNAO1 antibody (AB154001)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GNAO1 antibody (ab154001) at 1/10000 dilution

Lane 1:

Mouse brain tissue lysates at 50 µg

Lane 2:

Mouse lung tissue lysates at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40 kDa

false

Western blot - Anti-GNAO1 antibody (AB154001)
  • WB

Supplier Data

Western blot - Anti-GNAO1 antibody (AB154001)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GNAO1 antibody (ab154001) at 1/10000 dilution

Lane 1:

Rat brain tissue lysates at 50 µg

Lane 2:

Rat lung tissue lysates at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40 kDa

false

Western blot - Anti-GNAO1 antibody (AB154001)
  • WB

Supplier Data

Western blot - Anti-GNAO1 antibody (AB154001)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GNAO1 antibody (ab154001) at 1/5000 dilution

All lanes:

Adult zebrafish whole tissue lysate at 30 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Zebrafish, Human

Applications

IHC-P, ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human GNAO1 aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

P09471

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNAO1 also known as Guanine nucleotide-binding protein G(o) subunit alpha acts mechanically as part of the signaling processes within cells. It is a G protein alpha subunit that weighs around 41 kDa. The protein is widely expressed in neurons particularly affecting neurotransmitter signal transduction in brain regions like the cortex and hippocampus. GNAO1 is a part of the heterotrimeric G protein family that transduces signals from receptors on the cell surface to internal effectors playing a central role in signal processing.
Biological function summary

GNAO1 regulates the activity of various downstream effectors contributing to multiple cellular responses. It partners with beta and gamma subunits in forming a heterotrimeric G protein complex. The GNAO1's impact includes modulating neurotransmitter release influencing neuronal excitability and controlling other cellular processes like gene transcription. This protein's involvement in these processes highlights its necessity for normal neurological function and synaptic signaling.

Pathways

GNAO1 engages in critical molecular pathways such as the G protein-coupled receptor (GPCR) signaling pathway and the MAPK signaling pathway. It acts in concert with proteins like GNAI1 and GNB1 within these pathways contributing to a variety of cellular responses by transmitting signals received from extracellular ligands to initiate intracellular actions. GNAO1's activity in these pathways ensures proper signaling cascade required for normal cellular responses.

Mutations or dysfunctions of GNAO1 can lead to neurological conditions such as epileptic encephalopathy and movement disorders like dystonia. It relates closely to disorders through its interaction with proteins like GNB1 which also influence similar neurological diseases. These associations underline GNAO1's significance in maintaining neurological health shedding light on potential therapeutic targets for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 29925951). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (By similarity). Signaling by an activated GPCR promotes GDP release and GTP binding (By similarity). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (By similarity). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (By similarity). Signaling is mediated via effector proteins, such as adenylate cyclase (By similarity). Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (By similarity).
See full target information GNAO1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:1967-1984 PubMed40704638

2025

Developmental regulation of GABA receptors and downstream molecules in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Aguado,Rocío Alfaro-Ruiz,María Llanos Martínez-Poyato,Ana Esther Moreno-Martínez,Sebastián García-Madrona,Alberto Roldán-Sastre,Pablo Alonso-Gómez,Miriam Fernández,Ricardo Puertas-Avendaño,Ryuichi Shigemoto,Kirill A Martemyanov,Rafael Luján

Acta neuropathologica communications 12:24 PubMed38331815

2024

The Schwann cell-specific G-protein Gαo (Gnao1) is a cell-intrinsic controller contributing to the regulation of myelination in peripheral nerve system.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Xu,Qianqian Peng,Jieyi Cai,Jianghong Shangguan,Wenfeng Su,Gang Chen,Hualin Sun,Changlai Zhu,Yun Gu

Journal of inflammation research 16:5677-5695 PubMed38050561

2023

Identification of a Novel Target Implicated in Chronic Obstructive Sleep Apnea-Related Atrial Fibrillation by Integrative Analysis of Transcriptome and Proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Shen,Junqing Liang,Manzeremu Rejiepu,Ping Yuan,Jie Xiang,Yankai Guo,Jiasuoer Xiaokereti,Ling Zhang,Baopeng Tang

iScience 26:106701 PubMed37207277

2023

Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Han H Hsu,Greta Pintacuda,Ruize Liu,Eugeniu Nacu,April Kim,Kalliopi Tsafou,Natalie Petrossian,William Crotty,Jung Min Suh,Jackson Riseman,Jacqueline M Martin,Julia C Biagini,Daya Mena,Joshua K T Ching,Edyta Malolepsza,Taibo Li,Tarjinder Singh,Tian Ge,Shawn B Egri,Benjamin Tanenbaum,Caroline R Stanclift,Annie M Apffel,Steven A Carr,Monica Schenone,Jake Jaffe,Nadine Fornelos,Hailiang Huang,Kevin C Eggan,Kasper Lage

Frontiers in neuroanatomy 15:686279 PubMed34248508

2021

Cellular Diversity and Differential Subcellular Localization of the G-Protein G Subunit in the Mouse Cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Roldán-Sastre,Carolina Aguado,Alejandro Martín-Belmonte,Rocío Alfaro-Ruiz,Ana Esther Moreno-Martínez,Rafael Luján
View all publications
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