AB113429

Anti-GNAQ antibody

Name: Anti-GNAQ antibody (ab113429) | Abcam
Brand: Abcam Limited
SKU: AB113429
Price: 545 USD
Availability: InStock

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(1 Publication)

Goat Polyclonal GNAQ antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GNAQ aa 150-200.

View Alternative Names

GAQ, GNAQ, Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein alpha-q

2 Images

Supplier Data

Western blot - Anti-GNAQ antibody (AB113429)

Primary incubation was 1 hour. Detected using chemiluminescence.

All lanes:

Western blot - Anti-GNAQ antibody (ab113429) at 0.1 µg/mL

Lane 1:

Mouse testis tissue lysate in RIPA buffer at 35 µg

Lane 2:

Rat testis tissue lysate in RIPA buffer at 35 µg

Predicted band size: 42 kDa

Observed band size: 40 kDa

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Supplier Data

Western blot - Anti-GNAQ antibody (AB113429)

Primary incubation was 1 hour. Detected using chemiluminescence.

All lanes:

Western blot - Anti-GNAQ antibody (ab113429) at 0.1 µg/mL

Lane 1:

Pig colon tissue lysate in RIPA buffer at 35 µg

Lane 2:

Pig spleen tissue lysate in RIPA buffer at 35 µg

Predicted band size: 42 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GNAQ aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P50148

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "Primary incubation time: 1 hour.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "Primary incubation time: 1 hour.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "Primary incubation time: 1 hour.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab113429 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNAQ also known as Guanine nucleotide-binding protein G(q) subunit alpha plays a role in cell signaling as a modulator or transducer in various transmembrane signaling systems. With a molecular weight of approximately 42 kDa GNAQ belongs to the Gq family of G proteins. It is widely expressed in human tissues such as the brain heart and placenta. The protein's main mechanism involves binding to GTP and GDP leading it to switch between inactive GDP-bound and active GTP-bound states.

Biological function summary

GNAQ influences multiple cell processes mediating the activation of phospholipase C-beta a critical enzyme in hydrolyzing phosphatidylinositol 45-bisphosphate (PIP2) to inositol trisphosphate and diacylglycerol. This action leads to the release of calcium ions from intracellular stores. GNAQ acts as part of a heterotrimeric G protein complex which requires coordination with beta and gamma subunits to achieve full functional capability. Its activity is critical for processes like cell proliferation and differentiation.

Pathways

GNAQ notably participates in the phosphoinositide signaling pathway and calcium signaling pathway. In the phosphoinositide signaling pathway GNAQ facilitates the link between G protein-coupled receptors (GPCRs) and downstream effectors such as phospholipase C-beta. Its activity couples with proteins like GNA11 another member of the Gq family which often shares overlapping functions and roles within these pathways. The calcium signaling pathway involves GNAQ-induced release of calcium ions contributing to various cellular events.

GNAQ mutations link to uveal melanoma and Sturge-Weber syndrome. Studies show that these mutations activate the MAPK pathway independently promoting uncontrolled cell growth seen in uveal melanoma. GNA11 closely related to GNAQ also frequently undergoes similar mutations in uveal melanoma. In Sturge-Weber syndrome mutation of GNAQ leads to abnormal capillary growth and neurological issues evidencing its pathogenic role in vascular malformations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 37991948). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 37991948). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 37991948). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 37991948). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 37991948). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation : activates phospholipase C-beta : following GPCR activation, GNAQ activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed : 37991948). Required for platelet activation (By similarity). Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity (By similarity). Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed : 27852822). Together with GNA11, required for heart development (By similarity).

See full target information GNAQ

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular biochemistry 120:5766-5776 PubMed30320917

2018

The oncolytic virus H101 combined with GNAQ siRNA-mediated knockdown reduces uveal melanoma cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Yongyun Li,Jie He,Chun Qiu,Qingfeng Shang,Guanxiang Qian,Xianqun Fan,Shengfang Ge,Renbing Jia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GNAQ antibody

Western blot - Anti-GNAQ antibody (AB113429)

Western blot - Anti-GNAQ antibody (AB113429)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise