AB128060

Anti-GNAQ antibody

Name: Anti-GNAQ antibody (ab128060) | Abcam
Brand: Abcam Limited
SKU: AB128060
Price: 630 USD
Availability: InStock

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(8 Publications)

Goat Polyclonal GNAQ antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human GNAQ aa 150-200.

View Alternative Names

GAQ, GNAQ, Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein alpha-q

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAQ antibody (AB128060)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAQ antibody (AB128060)

ab128060, at 5 µg/ml, staining GNAQ in paraffin-embedded Human kidney tissue by Immunohistochemistry.

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Western blot - Anti-GNAQ antibody (AB128060)

All lanes:

Western blot - Anti-GNAQ antibody (ab128060) at 0.1 µg/mL

All lanes:

Rat Testis lysate (in RIPA buffer) at 35 µg

Predicted band size: 42 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GNAQ aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P50148

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNAQ also known as Guanine nucleotide-binding protein G(q) subunit alpha plays a role in cell signaling as a modulator or transducer in various transmembrane signaling systems. With a molecular weight of approximately 42 kDa GNAQ belongs to the Gq family of G proteins. It is widely expressed in human tissues such as the brain heart and placenta. The protein's main mechanism involves binding to GTP and GDP leading it to switch between inactive GDP-bound and active GTP-bound states.

Biological function summary

GNAQ influences multiple cell processes mediating the activation of phospholipase C-beta a critical enzyme in hydrolyzing phosphatidylinositol 45-bisphosphate (PIP2) to inositol trisphosphate and diacylglycerol. This action leads to the release of calcium ions from intracellular stores. GNAQ acts as part of a heterotrimeric G protein complex which requires coordination with beta and gamma subunits to achieve full functional capability. Its activity is critical for processes like cell proliferation and differentiation.

Pathways

GNAQ notably participates in the phosphoinositide signaling pathway and calcium signaling pathway. In the phosphoinositide signaling pathway GNAQ facilitates the link between G protein-coupled receptors (GPCRs) and downstream effectors such as phospholipase C-beta. Its activity couples with proteins like GNA11 another member of the Gq family which often shares overlapping functions and roles within these pathways. The calcium signaling pathway involves GNAQ-induced release of calcium ions contributing to various cellular events.

GNAQ mutations link to uveal melanoma and Sturge-Weber syndrome. Studies show that these mutations activate the MAPK pathway independently promoting uncontrolled cell growth seen in uveal melanoma. GNA11 closely related to GNAQ also frequently undergoes similar mutations in uveal melanoma. In Sturge-Weber syndrome mutation of GNAQ leads to abnormal capillary growth and neurological issues evidencing its pathogenic role in vascular malformations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 37991948). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 37991948). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 37991948). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 37991948). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 37991948). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation : activates phospholipase C-beta : following GPCR activation, GNAQ activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed : 37991948). Required for platelet activation (By similarity). Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity (By similarity). Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed : 27852822). Together with GNA11, required for heart development (By similarity).

See full target information GNAQ

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:11119 PubMed38750247

2024

Identification of Gα-vs-Gα-coupling determinants and development of a Gα-coupled designer GPCR.

Applications

Unspecified application

Species

Unspecified reactive species

Manae Tatsumi,Christian Cruz,Nozomi Kamakura,Riku Kuwabara,Gaku Nakamura,Tatsuya Ikuta,Ravinder Abrol,Asuka Inoue

Development (Cambridge, England) 150: PubMed37102682

2023

Stretch regulates alveologenesis and homeostasis via mesenchymal Gαq/11-mediated TGFβ2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda T Goodwin,Alison E John,Chitra Joseph,Anthony Habgood,Amanda L Tatler,Katalin Susztak,Matthew Palmer,Stefan Offermanns,Neil C Henderson,R Gisli Jenkins

Nature communications 13:487 PubMed35078997

2022

Heterotrimeric Gq proteins act as a switch for GRK5/6 selectivity underlying β-arrestin transducer bias.

Applications

Unspecified application

Species

Unspecified reactive species

Kouki Kawakami,Masataka Yanagawa,Suzune Hiratsuka,Misaki Yoshida,Yuki Ono,Michio Hiroshima,Masahiro Ueda,Junken Aoki,Yasushi Sako,Asuka Inoue

Genes to cells : devoted to molecular & cellular mechanisms 25:215-225 PubMed31989708

2020

OPN4 belongs to the photosensitive system of the human skin.

Applications

Unspecified application

Species

Unspecified reactive species

Junya Kusumoto,Makoto Takeo,Kazunobu Hashikawa,Takahide Komori,Takashi Tsuji,Hiroto Terashi,Shunsuke Sakakibara

Cell 177:1933-1947.e25 PubMed31160049

2019

Illuminating G-Protein-Coupling Selectivity of GPCRs.

Applications

Unspecified application

Species

Unspecified reactive species

Asuka Inoue,Francesco Raimondi,Francois Marie Ngako Kadji,Gurdeep Singh,Takayuki Kishi,Akiharu Uwamizu,Yuki Ono,Yuji Shinjo,Satoru Ishida,Nadia Arang,Kouki Kawakami,J Silvio Gutkind,Junken Aoki,Robert B Russell

The Journal of biological chemistry 292:5443-5456 PubMed28213525

2017

Conformational Profiling of the AT1 Angiotensin II Receptor Reflects Biased Agonism, G Protein Coupling, and Cellular Context.

Applications

Unspecified application

Species

Unspecified reactive species

Dominic Devost,Rory Sleno,Darlaine Pétrin,Alice Zhang,Yuji Shinjo,Rakan Okde,Junken Aoki,Asuka Inoue,Terence E Hébert

The Journal of investigative dermatology 137:727-736 PubMed27818281

2016

GPR56/ADGRG1 Activation Promotes Melanoma Cell Migration via NTF Dissociation and CTF-Mediated Gα/RhoA Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nien-Yi Chiang,Yen-Ming Peng,Horng-Heng Juang,Tse-Ching Chen,Hsiao-Lin Pan,Gin-Wen Chang,Hsi-Hsien Lin

Cellular and molecular life sciences : CMLS 72:165-79 PubMed24958088

2014

Using siRNA to define functional interactions between melanopsin and multiple G Protein partners.

Applications

IHC

Species

Unspecified reactive species

Steven Hughes,Aarti Jagannath,Doron Hickey,Silvia Gatti,Matthew Wood,Stuart N Peirson,Russell G Foster,Mark W Hankins

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