AB251282

Anti-GNAQ antibody [EPR17149] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal GNAQ antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.

View Alternative Names

GAQ, GNAQ, Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein alpha-q

2 Images

Supplier Data

Western blot - Anti-GNAQ antibody [EPR17149] - BSA and Azide free (AB251282)

This data was developed using ab199533, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GNAQ antibody [EPR17149] (<a href='/en-us/products/primary-antibodies/gnaq-antibody-epr17149-ab199533'>ab199533</a>) at 1/1000 dilution

Lane 1:

HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-GNAQ antibody [EPR17149] - BSA and Azide free (AB251282)

This data was developed using ab199533, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GNAQ antibody [EPR17149] (<a href='/en-us/products/primary-antibodies/gnaq-antibody-epr17149-ab199533'>ab199533</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat kidney lysate at 10 µg

Lane 6:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 7:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 8:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Unconjugated

Anti-GNAQ antibody [EPR17149]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17149

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 77% homology with GNA11. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251282 is the carrier-free version of ab199533.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNAQ also known as Guanine nucleotide-binding protein G(q) subunit alpha plays a role in cell signaling as a modulator or transducer in various transmembrane signaling systems. With a molecular weight of approximately 42 kDa GNAQ belongs to the Gq family of G proteins. It is widely expressed in human tissues such as the brain heart and placenta. The protein's main mechanism involves binding to GTP and GDP leading it to switch between inactive GDP-bound and active GTP-bound states.

Biological function summary

GNAQ influences multiple cell processes mediating the activation of phospholipase C-beta a critical enzyme in hydrolyzing phosphatidylinositol 45-bisphosphate (PIP2) to inositol trisphosphate and diacylglycerol. This action leads to the release of calcium ions from intracellular stores. GNAQ acts as part of a heterotrimeric G protein complex which requires coordination with beta and gamma subunits to achieve full functional capability. Its activity is critical for processes like cell proliferation and differentiation.

Pathways

GNAQ notably participates in the phosphoinositide signaling pathway and calcium signaling pathway. In the phosphoinositide signaling pathway GNAQ facilitates the link between G protein-coupled receptors (GPCRs) and downstream effectors such as phospholipase C-beta. Its activity couples with proteins like GNA11 another member of the Gq family which often shares overlapping functions and roles within these pathways. The calcium signaling pathway involves GNAQ-induced release of calcium ions contributing to various cellular events.

GNAQ mutations link to uveal melanoma and Sturge-Weber syndrome. Studies show that these mutations activate the MAPK pathway independently promoting uncontrolled cell growth seen in uveal melanoma. GNA11 closely related to GNAQ also frequently undergoes similar mutations in uveal melanoma. In Sturge-Weber syndrome mutation of GNAQ leads to abnormal capillary growth and neurological issues evidencing its pathogenic role in vascular malformations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 37991948). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 37991948). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 37991948). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 37991948). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 37991948). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation : activates phospholipase C-beta : following GPCR activation, GNAQ activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed : 37991948). Required for platelet activation (By similarity). Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity (By similarity). Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed : 27852822). Together with GNA11, required for heart development (By similarity).

See full target information GNAQ

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GNAQ antibody [EPR17149] - BSA and Azide free

Western blot - Anti-GNAQ antibody [EPR17149] - BSA and Azide free (AB251282)

Western blot - Anti-GNAQ antibody [EPR17149] - BSA and Azide free (AB251282)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise