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AB216129

Anti-GNAS antibody [7G6G5]

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(1 Publication)

Mouse Monoclonal GNAS antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GNAS1, GSP, GNAS, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Adenylate cyclase-stimulating G alpha protein

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-GNAS antibody [7G6G5] (AB216129)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GNAS antibody [7G6G5] (AB216129)

Immunofluorescent analaysis of HeLa cells labeling G protein alpha with ab216129 at 1/200 dilution. Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow Cytometry - Anti-GNAS antibody [7G6G5] (AB216129)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-GNAS antibody [7G6G5] (AB216129)

Flow cytometric analysis of MCF7 cells labeling G protein alpha with ab216129 at 1/200 dilution (green). Negative control (red).

Western blot - Anti-GNAS antibody [7G6G5] (AB216129)
  • WB

Supplier Data

Western blot - Anti-GNAS antibody [7G6G5] (AB216129)

Expected MW is 42.8 kDa

All lanes:

Western blot - Anti-GNAS antibody [7G6G5] (ab216129) at 1/500 dilution

All lanes:

Human G protein alpha (aa: 42-188) recombinant protein

Predicted band size: 46 kDa

false

Western blot - Anti-GNAS antibody [7G6G5] (AB216129)
  • WB

Supplier Data

Western blot - Anti-GNAS antibody [7G6G5] (AB216129)

All lanes:

Western blot - Anti-GNAS antibody [7G6G5] (ab216129) at 1/500 dilution

Lane 1:

non-transfected HEK293 cell lysate

Lane 2:

Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 46 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7G6G5

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GNAS protein also known as GNAS complex locus or guanine nucleotide-binding protein G(s) subunit alpha (Gαs) is a highly versatile target in cellular signaling. It has a molecular mass of approximately 45 kDa. Widely expressed across various tissues including those in the brain kidneys and endocrine glands GNAS protein plays an important role in transmitting signals from receptors on cell surfaces to effector systems inside cells.
Biological function summary

GNAS protein mediates the action of hormones and neurotransmitters by activating the cyclic AMP (cAMP) signaling pathway. It forms part of the G protein complex specifically the G protein-coupled receptors (GPCRs) where it acts as the alpha subunit interacting with beta and gamma subunits. This interaction facilitates the conversion of GDP to GTP leading to downstream activation of adenylate cyclase which increases intracellular cAMP levels and triggers various cellular responses.

Pathways

GNAS protein is pivotal in the cAMP signaling pathway and the MAPK/ERK pathway. Within these pathways it works closely with adenylate cyclase enzymes which further influence numerous physiological processes such as metabolism and cell growth. The protein’s activity also interlinks with other G proteins contributing to a wide range of cellular reactions and feedback mechanisms.

Mutations or dysregulation of the GNAS gene are associated with McCune-Albright syndrome and pseudohypoparathyroidism type 1a. McCune-Albright syndrome arises from somatic mutations in the GNAS gene that result in constitutively active Gαs protein leading to abnormal cellular proliferation. In pseudohypoparathyroidism the loss of GNAS protein function disrupts normal hormonal signaling causing resistance to parathyroid hormone (PTH) which impacts calcium and phosphate regulation in the body. The involvement of GNAS protein in these conditions highlights its key role in signal transduction and endocrine regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) (PubMed : 12391161, PubMed : 17110384, PubMed : 21488135, PubMed : 26206488, PubMed : 8702665, PubMed : 10200251). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed : 17110384, PubMed : 26206488, PubMed : 26206488, PubMed : 8702665). Functions downstream of beta-adrenergic receptors (PubMed : 21488135). Stimulates the Ras signaling pathway via RAPGEF2 (PubMed : 12391161).
See full target information GNAS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33300072

2020

miR‑654‑3p suppresses cell viability and promotes apoptosis by targeting RASAL2 in non‑small‑cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xiong,Shigang Xing,Zheng Dong,Lei Niu,Qinghua Xu,Yusheng Li,Pingyi Liu,Peixia Yang
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Product promise

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