AB101736

Anti-GNAS antibody

Name: Anti-GNAS antibody (ab101736) | Abcam
Brand: Abcam Limited
SKU: AB101736
Price: 535 USD
Availability: InStock

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(5 Publications)

Goat Polyclonal GNAS1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

View Alternative Names

GNAS1, GNAS, Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas, Adenylate cyclase-stimulating G alpha protein, Extra large alphas protein, XLalphas

2 Images

Supplier Data

Western blot - Anti-GNAS antibody (AB101736)

Lysates in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-GNAS antibody (ab101736) at 0.1 µg/mL

Lane 1:

Human cerebellum tissue lysate at 35 µg

Lane 2:

Mouse brain tissue lysate at 35 µg

Lane 3:

Rat brain tissue lysate at 35 µg

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Supplier Data

Western blot - Anti-GNAS antibody (AB101736)

Lysates in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-GNAS antibody (ab101736) at 0.3 µg/mL

All lanes:

KNRK cell lysate at 35 µg

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab101736 is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab101736 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GNAS protein also known as GNAS complex locus or guanine nucleotide-binding protein G(s) subunit alpha (Gαs) is a highly versatile target in cellular signaling. It has a molecular mass of approximately 45 kDa. Widely expressed across various tissues including those in the brain kidneys and endocrine glands GNAS protein plays an important role in transmitting signals from receptors on cell surfaces to effector systems inside cells.

Biological function summary

GNAS protein mediates the action of hormones and neurotransmitters by activating the cyclic AMP (cAMP) signaling pathway. It forms part of the G protein complex specifically the G protein-coupled receptors (GPCRs) where it acts as the alpha subunit interacting with beta and gamma subunits. This interaction facilitates the conversion of GDP to GTP leading to downstream activation of adenylate cyclase which increases intracellular cAMP levels and triggers various cellular responses.

Pathways

GNAS protein is pivotal in the cAMP signaling pathway and the MAPK/ERK pathway. Within these pathways it works closely with adenylate cyclase enzymes which further influence numerous physiological processes such as metabolism and cell growth. The protein’s activity also interlinks with other G proteins contributing to a wide range of cellular reactions and feedback mechanisms.

Mutations or dysregulation of the GNAS gene are associated with McCune-Albright syndrome and pseudohypoparathyroidism type 1a. McCune-Albright syndrome arises from somatic mutations in the GNAS gene that result in constitutively active Gαs protein leading to abnormal cellular proliferation. In pseudohypoparathyroidism the loss of GNAS protein function disrupts normal hormonal signaling causing resistance to parathyroid hormone (PTH) which impacts calcium and phosphate regulation in the body. The involvement of GNAS protein in these conditions highlights its key role in signal transduction and endocrine regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. XLas isoforms interact with the same set of receptors as Gnas isoforms.

See full target information GNAS

Additional targets

GNAS,GNAS

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 152: PubMed40207459

2025

Differential contribution of P73+ Cajal-Retzius cells and Reelin to cortical morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Vicente Elorriaga,Benoît Bouloudi,Elodie Delberghe,Yoann Saillour,Juliette S Morel,Patrick Azzam,Matthieu X Moreau,Rolf Stottmann,Nadia Bahi-Buisson,Alessandra Pierani,Nathalie Spassky,Frédéric Causeret

Cellular and molecular life sciences : CMLS 82:51 PubMed39833622

2025

Serotonin receptor 5-HT7 modulates inflammatory-associated functions of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Frauke S Bahr,Franziska E Müller,Martina Kasten,Nils Benen,Irina Sieve,Michaela Scherr,Christine S Falk,Denise Hilfiker-Kleiner,Melanie Ricke-Hoch,Evgeni Ponimaskin

International journal of molecular sciences 24: PubMed37686439

2023

Novel Correlation between TGF-β1/-β3 and Hormone Receptors in the Human Corneal Stroma.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander J Choi,Brenna S Hefley,Sarah E Nicholas,Rebecca L Cunningham,Dimitrios Karamichos

Sexual medicine 9:100390 PubMed34246178

2021

Overexpressing miR-122-5p Inhibits the Relaxation of Vaginal Smooth Muscle in Female Sexual Arousal Disorder by Targeting Vasoactive Intestinal Peptide Receptor 1.

Applications

Unspecified application

Species

Unspecified reactive species

Shengnan Cong,Tao Gui,Qinchuan Shi,Jingjing Zhang,Jingyi Feng,Lianjun Pan,Jiehua Ma,Aixia Zhang

Glia 69:872-889 PubMed33156956

2020

Serotonin receptor 4 regulates hippocampal astrocyte morphology and function.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska E Müller,Sophie K Schade,Volodymyr Cherkas,Laura Stopper,Björn Breithausen,Daniel Minge,Hristo Varbanov,Christian Wahl-Schott,Svitlana Antoniuk,Catia Domingos,Valérie Compan,Frank Kirchhoff,Christian Henneberger,Evgeni Ponimaskin,Andre Zeug

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GNAS antibody

Western blot - Anti-GNAS antibody (AB101736)

Western blot - Anti-GNAS antibody (AB101736)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (5)

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