AB273662

Anti-GNAS antibody [OTI7D7] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal GNAS antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

GNAS1, GSP, GNAS, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Adenylate cyclase-stimulating G alpha protein

2 Images

Supplier Data

Western blot - Anti-GNAS antibody [OTI7D7] - BSA and Azide free (AB273662)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

All lanes:

Western blot - Anti-GNAS antibody [OTI7D7] - BSA and Azide free (ab273662) at 1/500 dilution

Lane 1:

Jurkat cell extract at 35 µg

Lane 2:

MCF-7 cell extract at 35 µg

Lane 3:

HEK-293T cell extract at 35 µg

Lane 4:

HeLa cell extract at 35 µg

Lane 5:

HepG2 cell extract at 35 µg

Lane 6:

A549 cell extract at 35 µg

Lane 7:

K562 cell extract at 35 µg

Predicted band size: 46 kDa

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Supplier Data

Western blot - Anti-GNAS antibody [OTI7D7] - BSA and Azide free (AB273662)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

All lanes:

Western blot - Anti-GNAS antibody [OTI7D7] - BSA and Azide free (ab273662) at 1/2000 dilution

Lane 1:

HEK293T cells transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK293T cells transfected with pCMV6-ENTRY GNAS at 5 µg

Predicted band size: 46 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI7D7

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

pH: 7.3 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GNAS protein also known as GNAS complex locus or guanine nucleotide-binding protein G(s) subunit alpha (Gαs) is a highly versatile target in cellular signaling. It has a molecular mass of approximately 45 kDa. Widely expressed across various tissues including those in the brain kidneys and endocrine glands GNAS protein plays an important role in transmitting signals from receptors on cell surfaces to effector systems inside cells.

Biological function summary

GNAS protein mediates the action of hormones and neurotransmitters by activating the cyclic AMP (cAMP) signaling pathway. It forms part of the G protein complex specifically the G protein-coupled receptors (GPCRs) where it acts as the alpha subunit interacting with beta and gamma subunits. This interaction facilitates the conversion of GDP to GTP leading to downstream activation of adenylate cyclase which increases intracellular cAMP levels and triggers various cellular responses.

Pathways

GNAS protein is pivotal in the cAMP signaling pathway and the MAPK/ERK pathway. Within these pathways it works closely with adenylate cyclase enzymes which further influence numerous physiological processes such as metabolism and cell growth. The protein’s activity also interlinks with other G proteins contributing to a wide range of cellular reactions and feedback mechanisms.

Mutations or dysregulation of the GNAS gene are associated with McCune-Albright syndrome and pseudohypoparathyroidism type 1a. McCune-Albright syndrome arises from somatic mutations in the GNAS gene that result in constitutively active Gαs protein leading to abnormal cellular proliferation. In pseudohypoparathyroidism the loss of GNAS protein function disrupts normal hormonal signaling causing resistance to parathyroid hormone (PTH) which impacts calcium and phosphate regulation in the body. The involvement of GNAS protein in these conditions highlights its key role in signal transduction and endocrine regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) (PubMed : 12391161, PubMed : 17110384, PubMed : 21488135, PubMed : 26206488, PubMed : 8702665, PubMed : 10200251). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed : 17110384, PubMed : 26206488, PubMed : 26206488, PubMed : 8702665). Functions downstream of beta-adrenergic receptors (PubMed : 21488135). Stimulates the Ras signaling pathway via RAPGEF2 (PubMed : 12391161).

See full target information GNAS

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Product promise

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Anti-GNAS antibody [OTI7D7] - BSA and Azide free

Western blot - Anti-GNAS antibody [OTI7D7] - BSA and Azide free (AB273662)

Western blot - Anti-GNAS antibody [OTI7D7] - BSA and Azide free (AB273662)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise