JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB74059

Anti-GNAT1 antibody

5

(1 Review)

|

(10 Publications)

Rabbit Polyclonal GNAT1 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human GNAT1.

View Alternative Names

GNATR, GNAT1, Guanine nucleotide-binding protein G(t) subunit alpha-1, Transducin alpha-1 chain

1 Images
Western blot - Anti-GNAT1 antibody (AB74059)
  • WB

Unknown

Western blot - Anti-GNAT1 antibody (AB74059)

All lanes:

Western blot - Anti-GNAT1 antibody (ab74059) at 1/500 dilution

Lane 1:

extracts from COLO cells at 30 µg

Lane 2:

extracts from COLO cells at 30 µg with immunizing peptide

Predicted band size: 40 kDa

Observed band size: 40 kDa,48 kDa,70 kDa,85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GNAT1. The exact immunogen used to generate this antibody is proprietary information.

P11488

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500.00000 - 1/1000.00000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNAT1 also known as Guanine Nucleotide-Binding Protein G(t) Subunit Alpha-1 is an essential component of the visual process. This protein functions as the alpha-subunit of transducin an important player in the phototransduction cascade. GNAT1 has a molecular mass of approximately 40 kDa and is mainly expressed in photoreceptor cells of the retina. It participates in the conversion of photons into electrical signals by activating the cGMP phosphodiesterase enzyme upon stimulation by light-activated rhodopsin.
Biological function summary

GNAT1 plays a fundamental role in vision by mediating signal transduction in rods. It is part of the larger transducin protein complex which includes beta and gamma subunits. Upon activation by light GNAT1 binds to GTP and dissociates from the beta-gamma dimer to interact with downstream effectors initiating a series of reactions that lead to hyperpolarization of the photoreceptor cell membrane.

Pathways

GNAT1 functions within the phototransduction pathway an important system for visual signal processing. This pathway involves key interactions with proteins like rhodopsin and cGMP phosphodiesterase. In the presence of light GNAT1 converts GDP to GTP facilitating a cascade that results in the reduction of cGMP levels which closes cGMP-gated ion channels and alters the membrane potential effectively translating light stimuli into visual signals.

GNAT1 has significant associations with conditions affecting vision such as retinitis pigmentosa. Mutations in the GNAT1 gene can disrupt normal function and lead to defects in the phototransduction pathway contributing to progressive vision loss. Furthermore GNAT1's role in congenital stationary night blindness links it with proteins like rhodopsin and PDE6B emphasizing its involvement in maintaining proper visual function and its interactions that when disrupted result in ocular diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions as a signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (PubMed : 22190596). Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).
See full target information GNAT1
style
left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 9:17 PubMed33509294

2021

Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses.

Applications

Unspecified application

Species

Unspecified reactive species

James F Striebel,Brent Race,Jacqueline M Leung,Cindi Schwartz,Bruce Chesebro

Molecular therapy : the journal of the American So 28:830-844 PubMed32027843

2020

Repair of Retinal Degeneration following Ex Vivo Minicircle DNA Gene Therapy and Transplantation of Corrected Photoreceptor Progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Alona O Barnea-Cramer,Mandeep Singh,Dominik Fischer,Samantha De Silva,Michelle E McClements,Alun R Barnard,Robert E MacLaren

Nature communications 11:482 PubMed31980606

2020

Single AAV-mediated mutation replacement genome editing in limited number of photoreceptors restores vision in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Koji M Nishiguchi,Kosuke Fujita,Fuyuki Miya,Shota Katayama,Toru Nakazawa

Journal of cellular and molecular medicine 23:5176-5192 PubMed31251468

2019

The urokinase-type plasminogen activator system as drug target in retinitis pigmentosa: New pre-clinical evidence in the rd10 mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Maurizio Cammalleri,Massimo Dal Monte,Filippo Locri,Valeria Pecci,Mario De Rosa,Vincenzo Pavone,Paola Bagnoli

Journal of cellular physiology 234:18801-18812 PubMed30924157

2019

Disease mechanisms and neuroprotection by tauroursodeoxycholic acid in Rpgr knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Zhang,Uma Shahani,James Reilly,Xinhua Shu

Molecular therapy : the journal of the American So 26:2397-2406 PubMed30064895

2018

Retained Plasticity and Substantial Recovery of Rod-Mediated Visual Acuity at the Visual Cortex in Blind Adult Mice with Retinal Dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Koji M Nishiguchi,Kosuke Fujita,Naoyuki Tokashiki,Hiroshi Komamura,Sayaka Takemoto-Kimura,Hiroyuki Okuno,Haruhiko Bito,Toru Nakazawa

Scientific reports 7:8891 PubMed28827737

2017

Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Klaudia Szabó,Anna Énzsöly,Bulcsú Dékány,Arnold Szabó,Rozina I Hajdú,Tamás Radovits,Csaba Mátyás,Attila Oláh,Lenke K Laurik,Gábor M Somfai,Béla Merkely,Ágoston Szél,Ákos Lukáts

Cell and tissue research 356:49-63 PubMed24496510

2014

Immunocytochemical analysis of misplaced rhodopsin-positive cells in the developing rodent retina.

Applications

Unspecified application

Species

Unspecified reactive species

Klaudia Szabó,Arnold Szabó,Anna Enzsöly,Agoston Szél,Akos Lukáts

The Journal of biological chemistry 287:24092-102 PubMed22645143

2012

Leukemia inhibitory factor coordinates the down-regulation of the visual cycle in the retina and retinal-pigmented epithelium.

Applications

WB

Species

Mouse

Ana J Chucair-Elliott,Michael H Elliott,Jiangang Wang,Gennadiy P Moiseyev,Jian-Xing Ma,Luis E Politi,Nora P Rotstein,Shizuo Akira,Satoshi Uematsu,John D Ash

Experimental dermatology 20:568-71 PubMed21410772

2011

Phosphodiesterase inhibitors block the acceleration of skin permeability barrier repair by red light.

Applications

IHC-Fr

Species

Mouse

Makiko Goto,Kazuyuki Ikeyama,Moe Tsutsumi,Sumiko Denda,Mitsuhiro Denda
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com