Rabbit Polyclonal GNB1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 aa 50-300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Predicted | Predicted | Predicted |
Chicken | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Dog | Predicted | Predicted | Predicted |
Xenopus laevis | Predicted | Predicted | Predicted |
Xenopus tropicalis | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Dog, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Dog, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Dog, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Select an associated product type
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems (PubMed:29925951, PubMed:33762731, PubMed:34239069, PubMed:35610220, PubMed:35714614, PubMed:35835867, PubMed:36087581, PubMed:36989299, PubMed:37327704, PubMed:37935376, PubMed:37935377, PubMed:37963465, PubMed:37991948, PubMed:38168118, PubMed:38552625). The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (PubMed:29925951, PubMed:33762731, PubMed:34239069, PubMed:35610220, PubMed:35714614, PubMed:35835867, PubMed:36087581, PubMed:36989299, PubMed:37327704, PubMed:37935376, PubMed:37935377, PubMed:37963465, PubMed:38168118, PubMed:38552625).
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Transducin beta chain 1, GNB1
Rabbit Polyclonal GNB1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 aa 50-300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The GNB1 protein also known as Guanine nucleotide-binding protein subunit beta-1 is an important component in signal transduction across a variety of systems. With an approximate mass of 37 kDa GNB1 is expressed in numerous tissues throughout the human body including the central nervous system heart and spleen. Its role is to facilitate communication between receptor proteins on the cell surface and the interior machinery of the cell enabling efficient relay of external signals.
G-protein signaling inhibitors rely on the interaction of GNB1 within the cellular framework. GNB1 is part of a larger protein complex called heterotrimeric G-proteins. This complex includes three subunits: alpha beta and gamma where GNB1 typically partners with the gamma subunit to stabilize and enhance signal transduction. GNB1 modulates diverse biological processes by enabling proper functioning of neurotransmitter and hormone receptors.
GNB1 plays a role in the GPCR (G-protein-coupled receptor) signaling pathway which is one of the largest and most varied signal transduction pathways. Additionally GNB1 influences the MAPK signaling pathway which is important for regulating processes such as cell proliferation and differentiation. In these pathways GNB1 interacts closely with various proteins such as G-alpha subunits and mitogen-activated proteins ensuring precise and targeted signaling outcomes.
Mutations or dysregulation of GNB1 can lead to significant health issues such as intellectual disability and cancer. GNB1 abnormalities have been linked to an increased susceptibility to neurodevelopmental disorders where its interaction with G-alpha proteins becomes compromised. Additionally irregular GNB1 activity can influence oncogenic pathways contributing to the progression of certain types of cancer by altering cell signaling dynamics.
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10% SDS Page
All lanes: Western blot - Anti-GNB1 antibody (ab137635) at 1/5000 dilution
All lanes: Jurkat whole cell lysate at 30 µg
Predicted band size: 37 kDa
Immunohistochemical analysis of paraffin-embedded human Lung papillory Adeno Carcinoma, labelling GNB1 using ab137635 at 1/500 dilution.
Immunofluorescence analysis of methanol-fixed HeLa cells, labelling GNB1 using ab137635 at 1/200 dilution. Lower panel represents cells costained with Hoechst 33342.
10% SDS Page
All lanes: Western blot - Anti-GNB1 antibody (ab137635) at 1/10000 dilution
All lanes: Mouse brain whole cells lysate at 50 µg
Predicted band size: 37 kDa
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