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Rabbit Polyclonal GNB1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 aa 50-300.

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Images

Western blot - Anti-GNB1 antibody (AB137635), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB1 antibody (AB137635), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GNB1 antibody (AB137635), expandable thumbnail
  • Western blot - Anti-GNB1 antibody (AB137635), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link P62873

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Xenopus tropicalis
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Chicken, Cow, Dog, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/5000.00000 - 1/20000.00000

Notes

-

Species

Human

Dilution info

1/5000.00000 - 1/20000.00000

Notes

-

Predicted
Predicted

Species

Rat, Chicken, Cow, Dog, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Chicken, Cow, Dog, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems (PubMed:29925951, PubMed:33762731, PubMed:34239069, PubMed:35610220, PubMed:35714614, PubMed:35835867, PubMed:36087581, PubMed:36989299, PubMed:37327704, PubMed:37935376, PubMed:37935377, PubMed:37963465, PubMed:37991948, PubMed:38168118, PubMed:38552625). The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (PubMed:29925951, PubMed:33762731, PubMed:34239069, PubMed:35610220, PubMed:35714614, PubMed:35835867, PubMed:36087581, PubMed:36989299, PubMed:37327704, PubMed:37935376, PubMed:37935377, PubMed:37963465, PubMed:38168118, PubMed:38552625).

Alternative names

Recommended products

Rabbit Polyclonal GNB1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 aa 50-300.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link P62873
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The GNB1 protein also known as Guanine nucleotide-binding protein subunit beta-1 is an important component in signal transduction across a variety of systems. With an approximate mass of 37 kDa GNB1 is expressed in numerous tissues throughout the human body including the central nervous system heart and spleen. Its role is to facilitate communication between receptor proteins on the cell surface and the interior machinery of the cell enabling efficient relay of external signals.

Biological function summary

G-protein signaling inhibitors rely on the interaction of GNB1 within the cellular framework. GNB1 is part of a larger protein complex called heterotrimeric G-proteins. This complex includes three subunits: alpha beta and gamma where GNB1 typically partners with the gamma subunit to stabilize and enhance signal transduction. GNB1 modulates diverse biological processes by enabling proper functioning of neurotransmitter and hormone receptors.

Pathways

GNB1 plays a role in the GPCR (G-protein-coupled receptor) signaling pathway which is one of the largest and most varied signal transduction pathways. Additionally GNB1 influences the MAPK signaling pathway which is important for regulating processes such as cell proliferation and differentiation. In these pathways GNB1 interacts closely with various proteins such as G-alpha subunits and mitogen-activated proteins ensuring precise and targeted signaling outcomes.

Associated diseases and disorders

Mutations or dysregulation of GNB1 can lead to significant health issues such as intellectual disability and cancer. GNB1 abnormalities have been linked to an increased susceptibility to neurodevelopmental disorders where its interaction with G-alpha proteins becomes compromised. Additionally irregular GNB1 activity can influence oncogenic pathways contributing to the progression of certain types of cancer by altering cell signaling dynamics.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-GNB1 antibody (ab137635), expandable thumbnail

    Western blot - Anti-GNB1 antibody (ab137635)

    10% SDS Page

    All lanes: Western blot - Anti-GNB1 antibody (ab137635) at 1/5000 dilution

    All lanes: Jurkat whole cell lysate at 30 µg

    Predicted band size: 37 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB1 antibody (ab137635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB1 antibody (ab137635)

    Immunohistochemical analysis of paraffin-embedded human Lung papillory Adeno Carcinoma, labelling GNB1 using ab137635 at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-GNB1 antibody (ab137635), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GNB1 antibody (ab137635)

    Immunofluorescence analysis of methanol-fixed HeLa cells, labelling GNB1 using ab137635 at 1/200 dilution. Lower panel represents cells costained with Hoechst 33342.

  • Western blot - Anti-GNB1 antibody (ab137635), expandable thumbnail

    Western blot - Anti-GNB1 antibody (ab137635)

    10% SDS Page

    All lanes: Western blot - Anti-GNB1 antibody (ab137635) at 1/10000 dilution

    All lanes: Mouse brain whole cells lysate at 50 µg

    Predicted band size: 37 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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