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AB137635

Anti-GNB1 antibody

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(6 Publications)

Rabbit Polyclonal GNB1 antibody. Suitable for IHC-P, WB, IP, ICC/IF and reacts with Human, Mouse, Zebrafish samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GNB1 aa 50-300.

View Alternative Names

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Transducin beta chain 1, GNB1

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-GNB1 antibody (AB137635)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GNB1 antibody (AB137635)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling GNB1 protein at cytoplasm using ab137635 at a 1/500 dilution (Green). FIS1, a mitochondria marker, stained by an anti-FIS1 antibody at a 1/20000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB1 antibody (AB137635)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB1 antibody (AB137635)

Immunohistochemical analysis of paraffin-embedded human Lung papillory Adeno Carcinoma, labelling GNB1 using ab137635 at 1/500 dilution.

Immunoprecipitation - Anti-GNB1 antibody (AB137635)
  • IP

Supplier Data

Immunoprecipitation - Anti-GNB1 antibody (AB137635)

Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 µg of GNB1 antibody. Western blot analysis was performed using GNB1 antibody.

All lanes:

Immunoprecipitation - Anti-GNB1 antibody (ab137635) at 5 µg

Lane 1:

293T whole cell extracts

Lane 2:

293T whole cell extracts with Control IgG

Lane 3:

293T whole cell extracts (+)

Secondary

All lanes:

Anti rabbit IgG antibody

Predicted band size: 37 kDa

false

Western blot - Anti-GNB1 antibody (AB137635)
  • WB

Supplier Data

Western blot - Anti-GNB1 antibody (AB137635)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GNB1 antibody (ab137635) at 1/5000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 37 kDa

false

Western blot - Anti-GNB1 antibody (AB137635)
  • WB

Unknown

Western blot - Anti-GNB1 antibody (AB137635)

10% SDS Page

All lanes:

Western blot - Anti-GNB1 antibody (ab137635) at 1/10000 dilution

All lanes:

Mouse brain whole cells lysate at 50 µg

Predicted band size: 37 kDa

false

Western blot - Anti-GNB1 antibody (AB137635)
  • WB

Supplier Data

Western blot - Anti-GNB1 antibody (AB137635)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GNB1 antibody (ab137635) at 1/10000 dilution

Lane 1:

Zebrafish brain tissue extracts at 30 µg

Lane 2:

Zebrafish eye tissue extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 37 kDa

false

Western blot - Anti-GNB1 antibody (AB137635)
  • WB

Supplier Data

Western blot - Anti-GNB1 antibody (AB137635)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GNB1 antibody (ab137635) at 1/10000 dilution

Lane 1:

Zebrafish brain tissue extracts at 30 µg

Lane 2:

Zebrafish eye tissue extracts at 30 µg

Lane 3:

Whole zebrafish tissue extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Zebrafish

Applications

ICC/IF, WB, IP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GNB1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

P62873

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GNB1 protein also known as Guanine nucleotide-binding protein subunit beta-1 is an important component in signal transduction across a variety of systems. With an approximate mass of 37 kDa GNB1 is expressed in numerous tissues throughout the human body including the central nervous system heart and spleen. Its role is to facilitate communication between receptor proteins on the cell surface and the interior machinery of the cell enabling efficient relay of external signals.
Biological function summary

G-protein signaling inhibitors rely on the interaction of GNB1 within the cellular framework. GNB1 is part of a larger protein complex called heterotrimeric G-proteins. This complex includes three subunits: alpha beta and gamma where GNB1 typically partners with the gamma subunit to stabilize and enhance signal transduction. GNB1 modulates diverse biological processes by enabling proper functioning of neurotransmitter and hormone receptors.

Pathways

GNB1 plays a role in the GPCR (G-protein-coupled receptor) signaling pathway which is one of the largest and most varied signal transduction pathways. Additionally GNB1 influences the MAPK signaling pathway which is important for regulating processes such as cell proliferation and differentiation. In these pathways GNB1 interacts closely with various proteins such as G-alpha subunits and mitogen-activated proteins ensuring precise and targeted signaling outcomes.

Mutations or dysregulation of GNB1 can lead to significant health issues such as intellectual disability and cancer. GNB1 abnormalities have been linked to an increased susceptibility to neurodevelopmental disorders where its interaction with G-alpha proteins becomes compromised. Additionally irregular GNB1 activity can influence oncogenic pathways contributing to the progression of certain types of cancer by altering cell signaling dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems (PubMed : 29925951, PubMed : 33762731, PubMed : 34239069, PubMed : 35610220, PubMed : 35714614, PubMed : 35835867, PubMed : 36087581, PubMed : 36989299, PubMed : 37327704, PubMed : 37935376, PubMed : 37935377, PubMed : 37963465, PubMed : 37991948, PubMed : 38168118, PubMed : 38552625). The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (PubMed : 29925951, PubMed : 33762731, PubMed : 34239069, PubMed : 35610220, PubMed : 35714614, PubMed : 35835867, PubMed : 36087581, PubMed : 36989299, PubMed : 37327704, PubMed : 37935376, PubMed : 37935377, PubMed : 37963465, PubMed : 38168118, PubMed : 38552625).
See full target information GNB1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer 23:239 PubMed39449040

2024

A multidimensional recommendation framework for identifying biological targets to aid the diagnosis and treatment of liver metastasis in patients with colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Qi,Na Gao,Jia Li,Chenfei Zhou,Jinling Jiang,Bin Zhou,Liting Guo,Xiaohui Feng,Jun Ji,Qu Cai,Liu Yang,Rongjia Zhu,Xinyi Que,Junwei Wu,Wenqi Xi,Wenxing Qin,Jun Zhang

iScience 26:107534 PubMed37670789

2023

Inhibition of FNDC1 suppresses gastric cancer progression by interfering with Gβγ-VEGFR2 complex formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Lu,Panpan Huang,Xueliang Zeng,Wenyu Liu,Rui Zhao,Jing Li,Gaolu Cao,Yaqiong Hu,Qiuxiang Xiao,Meng Wu,Weicai Huang,Xuerui Tang,Xiaojian Liu,Hulai Wei

The Journal of biological chemistry 299:102880 PubMed36626984

2023

Disruption of the interaction between mutationally activated Gα and Gβγ attenuates aberrant signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna L Aumiller,Philip B Wedegaertner

Bioengineered 13:5638-5652 PubMed35193469

2022

AFAP1 antisense RNA 1 promotes retinoblastoma progression by sponging microRNA miR-545-3p that targets G protein subunit beta 1.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Tang,Li Zhang,Jing Li,Yu Guan

Experimental & molecular medicine 53:894-906 PubMed34012023

2021

PAX8-AS1 knockdown facilitates cell growth and inactivates autophagy in osteoblasts via the miR-1252-5p/GNB1 axis in osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Caiqiang Huang,Runguang Li,Changsheng Yang,Rui Ding,Qingchu Li,Denghui Xie,Rongkai Zhang,Yiyan Qiu

Journal of immunology (Baltimore, Md. : 1950) 202:1942-1947 PubMed30777924

2019

Cutting Edge: G Protein Subunit β 1 Negatively Regulates NLRP3 Inflammasome Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Tomohiko Murakami,Lerdluck Ruengsinpinya,Eriko Nakamura,Yoshifumi Takahata,Kenji Hata,Hiroaki Okae,Shun'ichiro Taniguchi,Masafumi Takahashi,Riko Nishimura
View all publications
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Product promise

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