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AB3433

Anti-GNB1 antibody

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(1 Publication)

Rabbit Polyclonal GNB1 antibody. Suitable for WB and reacts with Rat, Human, Sheep samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GNB1 aa 1-50.

View Alternative Names

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Transducin beta chain 1, GNB1

3 Images
Western blot - Anti-GNB1 antibody (AB3433)
  • WB

Supplier Data

Western blot - Anti-GNB1 antibody (AB3433)

Detection : chemiluminescence.

All lanes:

Western blot - Anti-GNB1 antibody (ab3433) at 2 µg/mL

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with skimmed milk

Lane 2:

Rat Brain with skimmed milk

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 37 kDa

false

Western blot - Anti-GNB1 antibody (AB3433)
  • WB

Unknown

Western blot - Anti-GNB1 antibody (AB3433)

Western blot of Tr beta on sheep retinal extracts using ab3433. Western blot of GNB1 on sheep retinal extracts using ab3433.

All lanes:

Western blot - Anti-GNB1 antibody (ab3433)

Predicted band size: 37 kDa

false

Western blot - Anti-GNB1 antibody (AB3433)
  • WB

Supplier Data

Western blot - Anti-GNB1 antibody (AB3433)

Detection : chemiluminescence.

All lanes:

Western blot - Anti-GNB1 antibody (ab3433) at 1 µg/mL

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Lane 3:

MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 4:

T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 30 µg

Lane 5:

tissue extract of Rat Brain at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 37 kDa

Observed band size: ~37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Sheep, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GNB1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P62873

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" }, "Amphibian": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GNB1 protein also known as Guanine nucleotide-binding protein subunit beta-1 is an important component in signal transduction across a variety of systems. With an approximate mass of 37 kDa GNB1 is expressed in numerous tissues throughout the human body including the central nervous system heart and spleen. Its role is to facilitate communication between receptor proteins on the cell surface and the interior machinery of the cell enabling efficient relay of external signals.
Biological function summary

G-protein signaling inhibitors rely on the interaction of GNB1 within the cellular framework. GNB1 is part of a larger protein complex called heterotrimeric G-proteins. This complex includes three subunits: alpha beta and gamma where GNB1 typically partners with the gamma subunit to stabilize and enhance signal transduction. GNB1 modulates diverse biological processes by enabling proper functioning of neurotransmitter and hormone receptors.

Pathways

GNB1 plays a role in the GPCR (G-protein-coupled receptor) signaling pathway which is one of the largest and most varied signal transduction pathways. Additionally GNB1 influences the MAPK signaling pathway which is important for regulating processes such as cell proliferation and differentiation. In these pathways GNB1 interacts closely with various proteins such as G-alpha subunits and mitogen-activated proteins ensuring precise and targeted signaling outcomes.

Mutations or dysregulation of GNB1 can lead to significant health issues such as intellectual disability and cancer. GNB1 abnormalities have been linked to an increased susceptibility to neurodevelopmental disorders where its interaction with G-alpha proteins becomes compromised. Additionally irregular GNB1 activity can influence oncogenic pathways contributing to the progression of certain types of cancer by altering cell signaling dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems (PubMed : 29925951, PubMed : 33762731, PubMed : 34239069, PubMed : 35610220, PubMed : 35714614, PubMed : 35835867, PubMed : 36087581, PubMed : 36989299, PubMed : 37327704, PubMed : 37935376, PubMed : 37935377, PubMed : 37963465, PubMed : 37991948, PubMed : 38168118, PubMed : 38552625). The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (PubMed : 29925951, PubMed : 33762731, PubMed : 34239069, PubMed : 35610220, PubMed : 35714614, PubMed : 35835867, PubMed : 36087581, PubMed : 36989299, PubMed : 37327704, PubMed : 37935376, PubMed : 37935377, PubMed : 37963465, PubMed : 38168118, PubMed : 38552625).
See full target information GNB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:995974 PubMed36203606

2022

Identification of immune-related endoplasmic reticulum stress genes in sepsis using bioinformatics and machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Gong,Yongbin Liu,Zhiyuan Tian,Min Zhang,Hejun Gao,Zhiyong Peng,Shuang Yin,Chi Wai Cheung,Youtan Liu
View all publications
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