Anti-GNB2 antibody [EP3262Y] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-GNB2 antibody [EP3262Y] - BSA and Azide free (AB247673)

This data was developed using the same antibody clone in a different buffer formulation (ab108504). Blocking and diluting buffer and concentration : 5% NFDM/TBST. Secondary antibody : incubated for 1h at 1 : 20,000 at room temperature (ab97051); incubated for 1h at 1 : 2000 at room temperature (Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG) Exposure time : Lane 1, 5 seconds. Lanes 2 and 3, 10 seconds. Lanes 4 and 5, 3 seconds.

All lanes:

Western blot - Anti-GNB2 antibody [EP3262Y] (<a href='/en-us/products/primary-antibodies/gnb2-antibody-ep3262y-ab108504'>ab108504</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

Mouse brain lysate at 20 µg

Lane 5:

Rat brain lysate at 20 µg

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• WB

Lab

Western blot - Anti-GNB2 antibody [EP3262Y] - BSA and Azide free (AB247673)

This data was developed using the same antibody clone in a different buffer formulation (ab108504).

Lanes 1-4 : Merged signal (red and green). Green - ab108504 observed at 35 kDa. Red - loading control ab7291 observed at 50 kDa.

ab108504 Anti-GNB2 antibody [EP3262Y] was shown to specifically react with GNB2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266528 (knockout cell lysate ab257453) was used. Wild-type and GNB2 knockout samples were subjected to SDS-PAGE. ab108504 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GNB2 antibody [EP3262Y] (<a href='/en-us/products/primary-antibodies/gnb2-antibody-ep3262y-ab108504'>ab108504</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

GNB2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human GNB2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-gnb2-knockout-hek-293t-cell-line-ab266528'>ab266528</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 37 kDa

Observed band size: 35 kDa

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