AB154866

Anti-GNB3 antibody [EPR8862]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal GNB3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, Transducin beta chain 3, GNB3

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GNB3 with ab154866 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

Immunohistochemical analysis of paraffin embedded Human normal heart tissue using ab154866 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154866 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154866 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154866 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] (AB154866)

Immunohistochemical analysis of paraffin embedded Human retina tissue using ab154866 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-GNB3 antibody [EPR8862] (AB154866)

WB

Unknown

Western blot - Anti-GNB3 antibody [EPR8862] (AB154866)

All lanes:

Western blot - Anti-GNB3 antibody [EPR8862] (ab154866) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

A673 cell lysate at 10 µg

Lane 4:

Human fetal lung tissue lysate at 10 µg

Predicted band size: 37 kDa

false

Carrier free

Anti-GNB3 antibody [EPR8862] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8862

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNB3 also known as Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 functions as an integral component of heterotrimeric G proteins. The protein has a mass of approximately 37 kDa. GNB3 is expressed in various tissues with significant expression in the brain and heart. It forms part of the G protein complex essential for transmitting signals from many hormones neurotransmitters and other signaling factors.

Biological function summary

GNB3 interacts with alpha and gamma subunits to mediate signal transduction from G protein-coupled receptors (GPCRs). It plays a role in sensory perception neurotransmission and regulation of ion channels and certain enzymes. GNB3 is part of the G protein complex which appears critical in maintaining cellular response and adaptation to external signals. The interaction with different G protein subunits enables diverse regulatory effects on cellular functions.

Pathways

GNB3 participates in the GPCR signaling pathway and the calcium signaling pathway. It interacts with G protein alpha subunits like GNAI1 and GNAS which are key players in these pathways. GPCR signaling affects numerous physiological processes involving aspects like cellular growth development and metabolic regulation. GNB3 contributes to converting the activated receptor signal into appropriate cellular responses.

Altered GNB3 function associates with essential hypertension and obesity. Studies show connections between specific GNB3 polymorphisms and these conditions sometimes indicating variations in response to antihypertensive treatments. GNB3 interacts with components of the signal transduction pathways like the protein PLCB1 which may further elucidate its role in disease mechanisms. Understanding these interactions could enhance therapeutic strategies targeting related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

See full target information Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2404841121 PubMed39348545

2024

TCA metabolism regulates DNA hypermethylation in LPS and -induced immune tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Abhimanyu,Santiago Carrero Longlax,Tomoki Nishiguchi,Malik Ladki,Daanish Sheikh,Amera L Martinez,Emily M Mace,Sandra L Grimm,Thaleia Caldwell,Alexandra Portillo Varela,Rajagopal V Sekhar,Anna M Mandalakas,Mandla Mlotshwa,Sibuse Ginidza,Jeffrey D Cirillo,Robert S Wallis,Mihai G Netea,Reinout van Crevel,Cristian Coarfa,Andrew R DiNardo

PeerJ 7:e6942 PubMed31143551

2019

Identification of RyR2-PBmice and the effects of transposon insertional mutagenesis of the RyR2 gene on cardiac function in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Wang,Chao Wang,Bo Wang,Qirui Shen,Leilei Qiu,Shuaijun Zou,Tao Wang,Guoyan Liu,Beilei Wang,Liming Zhang

Nucleic acid therapeutics 26:257-65 PubMed27028457

2016

Correction of the Pathogenic Alternative Splicing, Caused by the Common GNB3 c.825C>T Allele, Using a Novel, Antisense Morpholino.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan C P McGlinchey,Hemanth Tummala,Douglas H Lester

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com