AB251009

Anti-GNE antibody [EPR15059] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal GNE antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GLCNE, IBM2, GNE, Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-GlcNAc-2-epimerase/ManAc kinase

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-GNE antibody [EPR15059] - BSA and Azide free (AB251009)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GNE antibody [EPR15059] - BSA and Azide free (AB251009)

This data was developed using ab189927, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling GNE with ab189927 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.

Supplier Data

Immunoprecipitation - Anti-GNE antibody [EPR15059] - BSA and Azide free (AB251009)

This data was developed using ab189927, the same antibody clone in a different buffer formulation.

Western blot analysis of GNE in A549 cell lysate immunoprecipitated using ab189927 at 1/50 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-GNE antibody [EPR15059] - C-terminal (<a href='/en-us/products/primary-antibodies/gne-antibody-epr15059-c-terminal-ab189927'>ab189927</a>)

Predicted band size: 79 kDa

false

Supplier Data

Western blot - Anti-GNE antibody [EPR15059] - BSA and Azide free (AB251009)

This data was developed using ab189927, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GNE antibody [EPR15059] - C-terminal (<a href='/en-us/products/primary-antibodies/gne-antibody-epr15059-c-terminal-ab189927'>ab189927</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Lane 3:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 79 kDa

Observed band size: 75 kDa

false

Supplier Data

Western blot - Anti-GNE antibody [EPR15059] - BSA and Azide free (AB251009)

This data was developed using ab189927, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GNE antibody [EPR15059] - C-terminal (<a href='/en-us/products/primary-antibodies/gne-antibody-epr15059-c-terminal-ab189927'>ab189927</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 79 kDa

Observed band size: 75 kDa

false

Unconjugated

Anti-GNE antibody [EPR15059] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15059

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251009 is the carrier-free version of ab189927.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNE also called glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase is a bifunctional enzyme with a mass of approximately 82 kDa. This enzyme catalyzes the first two steps in sialic acid biosynthesis. GNE expression occurs in many tissues highest in liver and kidney and also in heart muscle and brain. Its bifunctional nature arises from two domain activities that facilitate the essential conversion of UDP-N-acetylglucosamine to N-acetylneuraminic acid a precursor for sialic acids.

Biological function summary

Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase acts as an important enzyme in sialic acid production acting alone and not part of a larger enzymatic complex. Sialic acids are terminal sugars on glycoproteins and glycolipids influencing cell-cell interaction signaling and stability. The enzyme's function affects cell surface structures impacting processes like cell migration and immune response.

Pathways

The enzyme GNE plays an important role in the sialylation pathway affecting various biological systems. This pathway involves the modification of glycoproteins and glycolipids critical for maintaining cellular communication. UDP-N-acetylglucosamine and N-acetylneuraminic acid are intermediates in pathways that also involve proteins like CMP-sialic acid transporter which mediates the transport of sialic acids into the Golgi apparatus for post-translational modification processes.

Defects in GNE are linked to conditions such as hereditary inclusion body myopathy (HIBM) an autosomal recessive muscle-wasting disorder. Another associated condition is sialuria a rare metabolic disorder tied to excessive levels of sialic acid. In HIBM mutations affect the enzyme’s kinase activity leading to reduced sialylation of glycoproteins. Research shows a relationship between GNE and proteins like sialin whose function as a transporter can impact the pathological manifestation of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Bifunctional enzyme that possesses both UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, and serves as the initiator of the biosynthetic pathway leading to the production of N-acetylneuraminic acid (NeuAc), a critical precursor in the synthesis of sialic acids. By catalyzing this pivotal and rate-limiting step in sialic acid biosynthesis, this enzyme assumes a pivotal role in governing the regulation of cell surface sialylation, playing a role in embryonic angiogenesis (PubMed : 10334995, PubMed : 11326336, PubMed : 14707127, PubMed : 16503651, PubMed : 2808337, PubMed : 38237079). Sialic acids represent a category of negatively charged sugars that reside on the surface of cells as terminal components of glycoconjugates and mediate important functions in various cellular processes, including cell adhesion, signal transduction, and cellular recognition (PubMed : 10334995, PubMed : 14707127).

See full target information GNE

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:454-462 PubMed31746378

2019

Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqing Hu,Wenyue Wang,Qing Ma,Tao Liu,Jiefeng Zhang,Jicun Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GNE antibody [EPR15059] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-GNE antibody [EPR15059] - BSA and Azide free (AB251009)

Immunoprecipitation - Anti-GNE antibody [EPR15059] - BSA and Azide free (AB251009)

Western blot - Anti-GNE antibody [EPR15059] - BSA and Azide free (AB251009)

Western blot - Anti-GNE antibody [EPR15059] - BSA and Azide free (AB251009)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise