Rabbit Polyclonal GNG2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GNG2.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human GNG2. Database link P59768
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Target data
Function
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems (PubMed:29925951, PubMed:33762731, PubMed:34239069, PubMed:35610220, PubMed:35714614, PubMed:35835867, PubMed:36087581, PubMed:36989299, PubMed:37327704, PubMed:37935376, PubMed:37935377, PubMed:37963465, PubMed:38168118, PubMed:38552625). The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (PubMed:29925951, PubMed:33762731, PubMed:34239069, PubMed:35610220, PubMed:35714614, PubMed:35835867, PubMed:36087581, PubMed:36989299, PubMed:37327704, PubMed:37935376, PubMed:37935377, PubMed:37963465, PubMed:38168118, PubMed:38552625).
Alternative names
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, G gamma-I, GNG2
Recommended products
Rabbit Polyclonal GNG2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GNG2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human GNG2. Database link P59768
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GNG2 also known as G-protein subunit gamma-2 is a small protein with a mass of approximately 8.5 kDa. It is a component of the heterotrimeric G-protein complex playing an essential role in signal transduction. GNG2 is expressed in various tissues including the brain heart and epithelial tissues. Its expression indicates a role in diverse cellular functions through its interaction with other G-protein subunits.
- Biological function summary
GNG2 forms part of the G-protein complex involved in transmitting signals from G-protein-coupled receptors (GPCRs) on the cell membrane to internal signaling pathways. This protein interacts with alpha and beta subunits to modulate downstream signaling cascades. The G-protein complex including GNG2 is essential for the activation of cellular responses to hormones neurotransmitters and environmental stimuli. It plays a significant role in regulating the activity of various effectors including adenylyl cyclase and phospholipase C.
- Pathways
GNG2 is directly involved in GPCR signaling pathways including the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for cell proliferation differentiation and survival. GNG2 interacts with other proteins like GNAS and PLCβ in these pathways contributing to the regulation of cellular responses and maintaining homeostasis. Its function in these pathways highlights its importance in coordinating complex cellular activities.
- Associated diseases and disorders
GNG2 has associations with cancer and cardiovascular diseases. Its interaction with proteins like RAS and PI3K suggests a role in tumorigenesis and cancer progression where dysregulated GPCR signaling could lead to abnormal cell growth. GNG2 also connects with proteins involved in cardiovascular functions such as those within the PI3K/AKT pathway indicating a potential link to cardiac disorders. Understanding GNG2's role in these diseases may offer insights into therapeutic targets for intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-GNG2 antibody (ab198225)
Gel: 10% SDS-PAGE
All lanes: Western blot - Anti-GNG2 antibody (ab198225) at 1/300 dilution
All lanes: Mouse paranephros tissue lysate at 40 µg
Developed using the ECL technique.
Predicted band size: 8 kDa
Exposure time: 5min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNG2 antibody (ab198225)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling GNG2 with ab198225 at 1/60 dilution.
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Product protocols
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