Rabbit Polyclonal GNG5 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GNG5.
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Publications
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- Biochemical and biophysical research communication 527:458-4652020MicroRNA-675-3p regulates IL-1β-stimulated human chondrocyte apoptosis and cartilage degradation by targeting GNG5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Fei Shen et. al.PubMed 32336544
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human GNG5. Database link P63218
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
Target data
Function
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Alternative names
GNGT5, GNG5, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-5
Recommended products
Rabbit Polyclonal GNG5 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GNG5.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human GNG5. Database link P63218
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity greater than 95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GNG5 also known as Guanine Nucleotide-Binding Protein G(I)/G(S)/G(O) Subunit Gamma-5 plays a role in the function of G protein-coupled receptors (GPCRs). It is a small protein subunit with a known mass of about 8 kDa. This protein is expressed in many tissues making it widely available for participation in various signaling pathways. The main function of GNG5 revolves around its association with the other G protein subunits in signal transduction where it is important in transmitting extracellular signals processed by receptors.
- Biological function summary
GNG5 functions as part of the larger G protein complex. It interacts with G alpha and G beta subunits to form the complete heterotrimeric G protein essential for GPCR signal transduction. By facilitating GDP-GTP exchange in the G alpha component GNG5 helps in activating downstream signaling cascades that influence physiological functions. Its involvement in cellular communication highlights its importance in biological processes like sensory perception and hormone responses.
- Pathways
GNG5 participates importantly in the G protein signaling pathway and the GPCR downstream pathway. During signal transduction GNG5 interacts with the G beta subunit to maintain the functional G protein state. This protein also has a relationship with other G proteins such as GNAI1 (Guanine Nucleotide Binding Protein Alpha Inhibiting Activity Polypeptide 1) reflecting its coordination in complex signaling pathways. These pathways are integral to key cellular responses and adaptability to external stimuli.
- Associated diseases and disorders
GNG5 has a connection to conditions like cancer and metabolic disorders. Aberrant expression or dysfunction of this protein could lead to disruptions in normal signaling contributing to oncogenic pathways. GNG5's interactions with other G proteins such as GNAS (Guanine Nucleotide Binding Protein Alpha Stimulating) highlight its involvement in disease-linked molecular mechanisms. Its role in these disorders makes it a potential target for therapeutic research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-GNG5 antibody (ab238835)
PC-3 (Human prostate adenocarcinoma cell line) cells stained for GNG5 (green) using ab238835 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNG5 antibody (ab238835)
Paraffin-embedded human liver cancer tissue stained for GNG5 using ab238835 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNG5 antibody (ab238835)
Paraffin-embedded human kidney tissue stained for GNG5 using ab238835 at 1/100 dilution in immunohistochemical analysis.
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