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AB245634

Anti-GNL1 antibody

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(2 Publications)

Rabbit Polyclonal GNL1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GNL1 aa 50-100.

View Alternative Names

HSR1, GNL1, Guanine nucleotide-binding protein-like 1, GTP-binding protein HSR1

2 Images
Immunoprecipitation - Anti-GNL1 antibody (AB245634)
  • IP

Unknown

Immunoprecipitation - Anti-GNL1 antibody (AB245634)

GNL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245634 at 10 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245634 at 0.4 μg/ml.

Lane 1 : ab245634 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-GNL1 antibody (ab245634)

Predicted band size: 69 kDa

false

Western blot - Anti-GNL1 antibody (AB245634)
  • WB

Supplier Data

Western blot - Anti-GNL1 antibody (AB245634)

All lanes:

Western blot - Anti-GNL1 antibody (ab245634) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 69 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human GNL1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P36915

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245634 was affinity purified using an epitope specific to GNL1 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNL1 also known as Nucleolar GTP-binding protein 1 is a protein with a molecular weight of approximately 67 kDa. It is mainly localized in the nucleolus of the cell where it is involved in ribosome biogenesis. The protein has a role in nucleolar processes with expression noted in various tissues which suggests a universal function across different cell types.
Biological function summary

GNL1 functions as a GTPase participating in the assembly and maturation of the ribosomal subunits. This role places it as a part of the Preribosomal Complex. GNL1 facilitates ribosomal RNA processing which is essential for proper protein synthesis within the cell. The protein's involvement in these nucleolar activities supports its significance in maintaining cellular homeostasis.

Pathways

GNL1 plays an integral role in the Nucleocytoplasmic Transport and Ribosome Biogenesis pathway. It works closely alongside proteins like Nucleophosmin (NPM1) during ribosome construction. The proper interaction of GNL1 with other nucleolar proteins ensures the seamless production of functional ribosomes which are important for cellular functions such as growth and division.

GNL1 has associations with certain types of cancer due to its critical role in cell proliferation. Altered expression levels of GNL1 may contribute to tumorigenesis particularly in cancers like leukemia. The protein's interaction with Tumor Suppressor Proteins such as p53 highlights its involvement in oncogenic pathways. Dysregulation of GNL1 and its complex network of interactions should be further studied to understand its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Possible regulatory or functional link with the histocompatibility cluster.
See full target information GNL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 32:1335-1346 PubMed39055884

2024

LncRNA HOTAIR promotes DNA damage repair and radioresistance by targeting ATR in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haiqing Hu,Hao Yang,Shuaishuai Fan,Xue Jia,Ying Zhao,Hongrui Li

RNA biology 18:1339-1353 PubMed33305682

2020

Guanine Nucleotide-Binding Protein-Like 1 (GNL1) binds RNA G-quadruplex structures in genes associated with Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Marc-Antoine Turcotte,Jean-Michel Garant,Hélène Cossette-Roberge,Jean-Pierre Perreault
View all publications
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