Rabbit Polyclonal GNL1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GNL1 aa 50-100.
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Publications
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- RNA biology 18:1339-13532020Guanine Nucleotide-Binding Protein-Like 1 (GNL1) binds RNA G-quadruplex structures in genes associated with Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarc-Antoine Turcotte,Jean-Michel Garant,Hélène Cossette-Roberge,Jean-Pierre PerreaultPubMed 33305682
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GNL1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P36915
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chimpanzee, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chimpanzee, Orangutan | Dilution info - | Notes - |
Target data
Function
Possible regulatory or functional link with the histocompatibility cluster.
Alternative names
HSR1, GNL1, Guanine nucleotide-binding protein-like 1, GTP-binding protein HSR1
Recommended products
Rabbit Polyclonal GNL1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GNL1 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GNL1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P36915
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab245634 was affinity purified using an epitope specific to GNL1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GNL1 also known as Nucleolar GTP-binding protein 1 is a protein with a molecular weight of approximately 67 kDa. It is mainly localized in the nucleolus of the cell where it is involved in ribosome biogenesis. The protein has a role in nucleolar processes with expression noted in various tissues which suggests a universal function across different cell types.
- Biological function summary
GNL1 functions as a GTPase participating in the assembly and maturation of the ribosomal subunits. This role places it as a part of the Preribosomal Complex. GNL1 facilitates ribosomal RNA processing which is essential for proper protein synthesis within the cell. The protein's involvement in these nucleolar activities supports its significance in maintaining cellular homeostasis.
- Pathways
GNL1 plays an integral role in the Nucleocytoplasmic Transport and Ribosome Biogenesis pathway. It works closely alongside proteins like Nucleophosmin (NPM1) during ribosome construction. The proper interaction of GNL1 with other nucleolar proteins ensures the seamless production of functional ribosomes which are important for cellular functions such as growth and division.
- Associated diseases and disorders
GNL1 has associations with certain types of cancer due to its critical role in cell proliferation. Altered expression levels of GNL1 may contribute to tumorigenesis particularly in cancers like leukemia. The protein's interaction with Tumor Suppressor Proteins such as p53 highlights its involvement in oncogenic pathways. Dysregulation of GNL1 and its complex network of interactions should be further studied to understand its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-GNL1 antibody (ab245634)
All lanes: Western blot - Anti-GNL1 antibody (ab245634) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 3min
Immunoprecipitation - Anti-GNL1 antibody (ab245634)
GNL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245634 at 10 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245634 at 0.4 μg/ml.
Lane 1: ab245634 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-GNL1 antibody (ab245634)
Predicted band size: 69 kDa
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