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AB170960

Anti-GNL2 antibody [EPR11479]

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(1 Publication)

Rabbit Recombinant Monoclonal GNL2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

NGP1, GNL2, Nucleolar GTP-binding protein 2, Autoantigen NGP-1

1 Images
Western blot - Anti-GNL2 antibody [EPR11479] (AB170960)
  • WB

Supplier Data

Western blot - Anti-GNL2 antibody [EPR11479] (AB170960)

All lanes:

Western blot - Anti-GNL2 antibody [EPR11479] (ab170960) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Lane 3:

Rat PC12 lysate at 10 µg

Lane 4:

Mouse NIH 3T3 lysate at 10 µg

Lane 5:

Ramos lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 84 kDa

true

  • Carrier free

    Anti-GNL2 antibody [EPR11479] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11479

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNL2 also known as nucleolar GTP-binding protein 2 functions mechanistically as a GTP-binding protein involved in ribosome biogenesis. With a mass of approximately 68 kDa GNL2 participates in nucleolar processes. Expression of GNL2 occurs broadly within tissues but shows notable presence in proliferative cells. It belongs to the MMR1/HSR1 GTP-binding protein family.
Biological function summary

GNL2 interacts as part of the nucleolar complex that contributes to ribosome assembly and maturation. It assists in the stabilization and processing of ribosomal RNA precursors aiding in the production of functional ribosomes necessary for protein synthesis. This connection highlights its role in cellular proliferation and homeostasis particularly within fast-dividing cells.

Pathways

GNL2 interacts with several important cellular processes beyond its primary role in ribosome biogenesis. It integrates into the cell cycle regulatory pathway influencing cell cycle progression and growth. GNL2 also shows involvement with proteins such as nucleolin and nucleophosmin partners essential for its function in ribosome maturation and the regulation of nucleolar structure.

GNL2 exerts influence on conditions associated with abnormal cell proliferation. It has a connection to cancers given its role in accelerating ribosome biogenesis and supporting high proliferation rates in tumor cells. GNL2's dysregulation links it with oncogenic processes often interacting with proteins like p53 an essential regulator in cancer pathways. Additionally aberrant GNL2 expression might relate to disorders involving disrupted cell cycle control.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation (PubMed : 32669547). May promote cell proliferation possibly by increasing p53/TP53 protein levels, and consequently those of its downstream product CDKN1A/p21, and decreasing RPL23A protein levels (PubMed : 26203195).
See full target information GNL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunology 164:754-765 PubMed34432883

2021

Transcriptomic landscape of skin lesions in cutaneous leishmaniasis reveals a strong CD8 T cell immunosenescence signature linked to immunopathology.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Henrique Fantecelle,Luciana Polaco Covre,Renan Garcia de Moura,Herbert Leonel de Matos Guedes,Camila Farias Amorim,Phillip Scott,David Mosser,Aloisio Falqueto,Arne N Akbar,Daniel Claudio Oliveira Gomes
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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