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AB249625

Anti-GNL2 antibody [EPR11479] - BSA and Azide free

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Rabbit Recombinant Monoclonal GNL2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.

View Alternative Names

NGP1, GNL2, Nucleolar GTP-binding protein 2, Autoantigen NGP-1

1 Images
Western blot - Anti-GNL2 antibody [EPR11479] - BSA and Azide free (AB249625)
  • WB

Supplier Data

Western blot - Anti-GNL2 antibody [EPR11479] - BSA and Azide free (AB249625)

This data was developed using ab170960, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GNL2 antibody [EPR11479] (<a href='/en-us/products/primary-antibodies/gnl2-antibody-epr11479-ab170960'>ab170960</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Lane 3:

Rat PC12 lysate at 10 µg

Lane 4:

Mouse NIH 3T3 lysate at 10 µg

Lane 5:

Ramos lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 84 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11479

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249625 is the carrier-free version of ab170960.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNL2 also known as nucleolar GTP-binding protein 2 functions mechanistically as a GTP-binding protein involved in ribosome biogenesis. With a mass of approximately 68 kDa GNL2 participates in nucleolar processes. Expression of GNL2 occurs broadly within tissues but shows notable presence in proliferative cells. It belongs to the MMR1/HSR1 GTP-binding protein family.
Biological function summary

GNL2 interacts as part of the nucleolar complex that contributes to ribosome assembly and maturation. It assists in the stabilization and processing of ribosomal RNA precursors aiding in the production of functional ribosomes necessary for protein synthesis. This connection highlights its role in cellular proliferation and homeostasis particularly within fast-dividing cells.

Pathways

GNL2 interacts with several important cellular processes beyond its primary role in ribosome biogenesis. It integrates into the cell cycle regulatory pathway influencing cell cycle progression and growth. GNL2 also shows involvement with proteins such as nucleolin and nucleophosmin partners essential for its function in ribosome maturation and the regulation of nucleolar structure.

GNL2 exerts influence on conditions associated with abnormal cell proliferation. It has a connection to cancers given its role in accelerating ribosome biogenesis and supporting high proliferation rates in tumor cells. GNL2's dysregulation links it with oncogenic processes often interacting with proteins like p53 an essential regulator in cancer pathways. Additionally aberrant GNL2 expression might relate to disorders involving disrupted cell cycle control.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation (PubMed : 32669547). May promote cell proliferation possibly by increasing p53/TP53 protein levels, and consequently those of its downstream product CDKN1A/p21, and decreasing RPL23A protein levels (PubMed : 26203195).
See full target information GNL2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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