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Rabbit Recombinant Monoclonal GNLY/Granulysin antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-101] (AB241333), expandable thumbnail
  • Western blot - Anti-GNLY/Granulysin antibody [EPR22110-101] (AB241333), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (AB241333), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (AB241333), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/4000
Notes

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

Alternative names

LAG2, NKG5, TLA519, GNLY, Granulysin, Lymphokine LAG-2, Protein NKG5, T-cell activation protein 519

Recommended products

Rabbit Recombinant Monoclonal GNLY/Granulysin antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22110-101
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Granulysin also known as GNLY is a cytolytic protein with a mass of approximately 9 kDa present in the granules of cytotoxic T lymphocytes and natural killer cells. This protein expressed in activated human immune cells plays a role in immune defense by creating pores in the target cell membranes which enables other cytotoxic molecules to enter and induce cell death. Researchers often utilize Granulysin as a GNLY marker to assess immune cell activity within the body.

Biological function summary

Granulysin contributes to the body's defense against bacterial fungal and parasitic infections. It acts independently but can also collaborate with other immune components to enhance its effects. Granulysin disrupts microbial membranes owing to its affinity to negatively charged lipids leading to pathogen elimination. This mechanism plays a critical role in the human body's broader immune response.

Pathways

Granulysin operates significantly within the immune response pathway by working in concert with perforin another cytolytic protein. Together they form a potent immune mechanism aimed at eliminating infected or malignant cells. Furthermore the interferon-gamma pathway closely influences Granulysin suggesting a connection to innate and adaptive immune responses.

Associated diseases and disorders

Granulysin shows association with various conditions most notably tuberculosis and leprosy. In these diseases the GNLY protein levels reflect the body's immune activity against the pathogens. Granulysin works alongside proteins such as perforin and granzymes whose involvement suggests possible diagnostic markers for understanding the immune response in these bacterial infections.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PBMC (human peripheral blood mononuclear cell) cells labeling GNLY/Granulysin with ab241333 at 1/250 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (Green) at 1/1000 dilution.

    Confocal image showing cytoplasmic staining in a part of human PBMC. The nuclear counterstain was DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used as a counterstain (Red).

  • Western blot - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333), expandable thumbnail

    Western blot - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333) at 1/1000 dilution

    All lanes: His-tagged human GNLY/Granulysin recombinant protein (aa23-145) 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 16 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling GNLY/Granulysin with ab241333 at 1/4000 dilution, followed by a ready to use Goat anti-Rabbit IgG H&L (HRP). The section was incubated with ab for 30 mins at 37°C. Sporadic staining on human spleen (PMID: 30151671) is observed, performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling GNLY/Granulysin with ab241333 at 1/4000 dilution, followed by a ready to use Goat anti-Rabbit IgG H&L (HRP). The section was incubated with ab for 30 mins at 37°C. Positive staining on lymphocytes of human endometrium (PMID: 26488136) is observed, performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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