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AB238460

Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal GNLY/Granulysin antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LAG2, NKG5, TLA519, GNLY, Granulysin, Lymphokine LAG-2, Protein NKG5, T-cell activation protein 519

1 Images
Flow Cytometry (Intracellular) - Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (AB238460)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (AB238460)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed, 0.1% Tween 20-permeabilized PBMCs (human peripheral blood mononuclear cell) cells labeling GNLY/Granulysin with ab223326 at 1/600 (right) compared with a Isotype control details (ab172730) (left).

Human PBMCs were stained with PE-conjugated anti-human CD56. Cells were then fixed with 2% PFA for 10min and permeabilized with 0.1% Tween-20, followed by intracellular staining with rabbit IgG (ab172730, left) or ab223326, right).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223326).

  • Carrier free

    Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (Detector)

  • Unconjugated

    Anti-GNLY/Granulysin antibody [EPR22110-128]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22110-128

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab238460 is the carrier-free version of ab223326.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granulysin also known as GNLY is a cytolytic protein with a mass of approximately 9 kDa present in the granules of cytotoxic T lymphocytes and natural killer cells. This protein expressed in activated human immune cells plays a role in immune defense by creating pores in the target cell membranes which enables other cytotoxic molecules to enter and induce cell death. Researchers often utilize Granulysin as a GNLY marker to assess immune cell activity within the body.
Biological function summary

Granulysin contributes to the body's defense against bacterial fungal and parasitic infections. It acts independently but can also collaborate with other immune components to enhance its effects. Granulysin disrupts microbial membranes owing to its affinity to negatively charged lipids leading to pathogen elimination. This mechanism plays a critical role in the human body's broader immune response.

Pathways

Granulysin operates significantly within the immune response pathway by working in concert with perforin another cytolytic protein. Together they form a potent immune mechanism aimed at eliminating infected or malignant cells. Furthermore the interferon-gamma pathway closely influences Granulysin suggesting a connection to innate and adaptive immune responses.

Granulysin shows association with various conditions most notably tuberculosis and leprosy. In these diseases the GNLY protein levels reflect the body's immune activity against the pathogens. Granulysin works alongside proteins such as perforin and granzymes whose involvement suggests possible diagnostic markers for understanding the immune response in these bacterial infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.
See full target information GNLY
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Brain, behavior, and immunity 80:247-254 PubMed30885840

2019

Methamphetamine-associated cognitive decline is attenuated by neutralizing IL-1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Arkadiusz Liśkiewicz,Marta Przybyła,Minseon Park,Daniela Liśkiewicz,Marta Nowacka-Chmielewska,Andrzej Małecki,Jarosław Barski,Joanna Lewin-Kowalik,Michal Toborek
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com