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Rabbit Recombinant Monoclonal GNLY/Granulysin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-GNLY/Granulysin antibody [EPR22110-180] - BSA and Azide free (Capture) (AB259451), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal GNLY/Granulysin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR22110-180

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab259451 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Granulysin. The recommended pair for sandwich ELISA is:
Capture: ab259451, Human Granulysin Capture Antibody (unconjugated)
Detector: Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (Detector) ab259452, Human Granulysin Detector Antibody (unconjugated)
The reference range value is 78.125 - 5000 pg/ml.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Granulysin also known as GNLY is a cytolytic protein with a mass of approximately 9 kDa present in the granules of cytotoxic T lymphocytes and natural killer cells. This protein expressed in activated human immune cells plays a role in immune defense by creating pores in the target cell membranes which enables other cytotoxic molecules to enter and induce cell death. Researchers often utilize Granulysin as a GNLY marker to assess immune cell activity within the body.

Biological function summary

Granulysin contributes to the body's defense against bacterial fungal and parasitic infections. It acts independently but can also collaborate with other immune components to enhance its effects. Granulysin disrupts microbial membranes owing to its affinity to negatively charged lipids leading to pathogen elimination. This mechanism plays a critical role in the human body's broader immune response.

Pathways

Granulysin operates significantly within the immune response pathway by working in concert with perforin another cytolytic protein. Together they form a potent immune mechanism aimed at eliminating infected or malignant cells. Furthermore the interferon-gamma pathway closely influences Granulysin suggesting a connection to innate and adaptive immune responses.

Associated diseases and disorders

Granulysin shows association with various conditions most notably tuberculosis and leprosy. In these diseases the GNLY protein levels reflect the body's immune activity against the pathogens. Granulysin works alongside proteins such as perforin and granzymes whose involvement suggests possible diagnostic markers for understanding the immune response in these bacterial infections.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Anti-GNLY/Granulysin antibody [EPR22110-180] - BSA and Azide free (Capture) (ab259451), expandable thumbnail

    Sandwich ELISA - Anti-GNLY/Granulysin antibody [EPR22110-180] - BSA and Azide free (Capture) (ab259451)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Human Granulysin ELISA Kit ab256402).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com