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AB203396

Anti-GNMT antibody

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(1 Publication)

Rabbit Polyclonal GNMT antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Glycine N-methyltransferase aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Glycine N-methyltransferase, GNMT

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNMT antibody (AB203396)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNMT antibody (AB203396)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling GNMT with ab203396 at 1/200 followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Glycine N-methyltransferase aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q14749

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Alternative Products

Primary Antibodies

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Anti-GNMT antibody

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Primary Antibodies

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Proteins & Peptides

AB79190

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNMT also known as Glycine N-methyltransferase functions by catalyzing the methyl transfer from S-adenosylmethionine (SAM) to glycine which results in the formation of N-methylglycine. This enzyme possesses a molecular mass of approximately 292 amino acids. GNMT expresses strongly in the liver but also occurs in other tissues like the pancreas. It maintains the balance of SAM and influences cellular methylation processes.
Biological function summary

GNMT plays a vital role in regulating the cellular methyl group pool. It lacks association with any complex; instead GNMT works independently to control the levels of SAM influencing numerous methylation reactions. Its activity helps in maintaining normal liver function and detoxification processes within the body. Furthermore GNMT impacts the metabolism of several amino acids.

Pathways

GNMT participates in the methionine cycle and folate metabolism pathways. In the methionine cycle GNMT regulates SAM levels alongside proteins such as methionine adenosyltransferase and S-adenosylhomocysteine hydrolase which influence methylation activities broadly. Through its action within the folate metabolism it connects indirectly with proteins like dihydrofolate reductase contributing to nucleic acid synthesis and cellular growth.

GNMT shows a significant association with liver-related diseases and certain cancers. Altered GNMT expression or activity links with liver dysfunction and hepatocellular carcinoma. Additionally in cancer GNMT dysregulation interacts with proteins like tumor suppressor p53 influencing cell proliferation and apoptosis. GNMT's effect on SAM levels affects global DNA methylation impacting gene expression and contributing to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy), a reaction regulated by the binding of 5-methyltetrahydrofolate. Plays an important role in the regulation of methyl group metabolism by regulating the ratio between S-adenosyl-L-methionine and S-adenosyl-L-homocysteine.
See full target information Glycine N-methyltransferase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 25:663-676.e6 PubMed30332646

2018

Sarcosine Is Uniquely Modulated by Aging and Dietary Restriction in Rodents and Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan O Walters,Esperanza Arias,Antonio Diaz,Emmanuel S Burgos,Fangxia Guan,Simoni Tiano,Kai Mao,Cara L Green,Yungping Qiu,Hardik Shah,Donghai Wang,Adam D Hudgins,Tahmineh Tabrizian,Valeria Tosti,David Shechter,Luigi Fontana,Irwin J Kurland,Nir Barzilai,Ana Maria Cuervo,Daniel E L Promislow,Derek M Huffman
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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