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AB224753

Anti-GNMT antibody

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(2 Publications)

Rabbit Polyclonal GNMT antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glycine N-methyltransferase aa 100-200.

View Alternative Names

Glycine N-methyltransferase, GNMT

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNMT antibody (AB224753)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNMT antibody (AB224753)

Paraffin-embedded human pancreas tissue stained for GNMT using ab224753 at 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GNMT antibody (AB224753)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GNMT antibody (AB224753)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for GNMT (green) using ab224753 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Glycine N-methyltransferase aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q14749

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNMT also known as Glycine N-methyltransferase functions by catalyzing the methyl transfer from S-adenosylmethionine (SAM) to glycine which results in the formation of N-methylglycine. This enzyme possesses a molecular mass of approximately 292 amino acids. GNMT expresses strongly in the liver but also occurs in other tissues like the pancreas. It maintains the balance of SAM and influences cellular methylation processes.
Biological function summary

GNMT plays a vital role in regulating the cellular methyl group pool. It lacks association with any complex; instead GNMT works independently to control the levels of SAM influencing numerous methylation reactions. Its activity helps in maintaining normal liver function and detoxification processes within the body. Furthermore GNMT impacts the metabolism of several amino acids.

Pathways

GNMT participates in the methionine cycle and folate metabolism pathways. In the methionine cycle GNMT regulates SAM levels alongside proteins such as methionine adenosyltransferase and S-adenosylhomocysteine hydrolase which influence methylation activities broadly. Through its action within the folate metabolism it connects indirectly with proteins like dihydrofolate reductase contributing to nucleic acid synthesis and cellular growth.

GNMT shows a significant association with liver-related diseases and certain cancers. Altered GNMT expression or activity links with liver dysfunction and hepatocellular carcinoma. Additionally in cancer GNMT dysregulation interacts with proteins like tumor suppressor p53 influencing cell proliferation and apoptosis. GNMT's effect on SAM levels affects global DNA methylation impacting gene expression and contributing to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy), a reaction regulated by the binding of 5-methyltetrahydrofolate. Plays an important role in the regulation of methyl group metabolism by regulating the ratio between S-adenosyl-L-methionine and S-adenosyl-L-homocysteine.
See full target information Glycine N-methyltransferase
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of hepatocellular carcinoma 11:95-111 PubMed38250306

2024

The Role of GNMT and MMP12 Expression in Determining TACE Efficacy: Validation at Transcription and Protein Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhao Cong,Chao Yang,Qi Cao,Jinrui Ren,Yingen Luo,Pei Yuan,Bo Zheng,Yu Liu,Hongcai Yang,Wendi Kang,Aixin Ou,Xiao Li

PloS one 15:e0242606 PubMed33211757

2020

Zinc deficiency induces abnormal development of the myocardium by promoting SENP5 overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Zhang,Cuancuan Wang,Dan Zhao,Xuhong Chen,Chunyan Zhang,Jun Zheng,Xiaozhi Liu
View all publications
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Product promise

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