Rabbit Polyclonal GNPDA1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GNPDA1 aa 100-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GNPDA1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P46926
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Cow | Predicted |
| Xenopus laevis | Predicted |
| Xenopus tropicalis | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Target data
Function
Catalyzes the reversible conversion of alpha-D-glucosamine 6-phosphate (GlcN-6P) into beta-D-fructose 6-phosphate (Fru-6P) and ammonium ion, a regulatory reaction step in de novo uridine diphosphate-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) biosynthesis via hexosamine pathway. Deamination is coupled to aldo-keto isomerization mediating the metabolic flux from UDP-GlcNAc toward Fru-6P. At high ammonium level can drive amination and isomerization of Fru-6P toward hexosamines and UDP-GlcNAc synthesis (PubMed:21807125, PubMed:26887390). Has a role in fine tuning the metabolic fluctuations of cytosolic UDP-GlcNAc and their effects on hyaluronan synthesis that occur during tissue remodeling (PubMed:26887390). Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo (By similarity).
Alternative names
GNPI, HLN, KIAA0060, GNPDA1, Glucosamine-6-phosphate isomerase 1, Glucosamine-6-phosphate deaminase 1, Oscillin, GNPDA 1, GlcN6P deaminase 1
Recommended products
Rabbit Polyclonal GNPDA1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GNPDA1 aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GNPDA1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P46926
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GNPDA1 also known as glucosamine-6-phosphate deaminase 1 is an enzyme that plays an important role in the hexosamine biosynthesis pathway. This protein has a molecular mass of approximately 33 kDa. GNPDA1 is expressed in various human tissues with higher levels noticeable in the kidney and liver. It catalyzes the conversion of glucosamine-6-phosphate into fructose-6-phosphate and ammonia a reaction important for cellular carbohydrate metabolism.
- Biological function summary
Glucosamine-6-phosphate deaminase 1 influences cell processes through its enzymatic activity. This enzyme functions as a monomer not requiring complex formation to exert its biological role. By contributing to the hexosamine biosynthetic pathway GNPDA1 affects key physiological functions including cell signaling and protein glycosylation. Its activity can influence cellular responses to nutrient availability and metabolic stress.
- Pathways
The enzyme glucosamine-6-phosphate deaminase 1 plays a role in the hexosamine biosynthetic pathway and glycolysis. These pathways are essential for cellular energy metabolism and protein modification processes. GNPDA1 interacts with related proteins such as fructose-6-phosphate which links it to broader metabolic networks involving glucose utilization and adaptation to metabolic demands.
- Associated diseases and disorders
Alterations in GNPDA1 activity have implications for metabolic conditions including cancer and diabetes. Abnormal expression or function of this enzyme can disrupt metabolic homeostasis influencing cancer cell metabolism due to altered hexosamine pathway flux. GNPDA1's relationship with proteins involved in metabolic regulation such as those in the insulin signaling pathway can contribute to disease progression and present potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNPDA1 antibody (ab253023)
Formalin-fixed, paraffin-embedded human kidney tissue stained for GNPDA1 with ab253023 at a 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNPDA1 antibody (ab253023)
Formalin-fixed, paraffin-embedded human endometrium tissue stained for GNPDA1 with ab253023 at a 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNPDA1 antibody (ab253023)
Formalin-fixed, paraffin-embedded human testis tissue stained for GNPDA1 with ab253023 at a 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNPDA1 antibody (ab253023)
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for GNPDA1 with ab253023 at a 1/20 dilution in immunohistochemical analysis.
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