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AB127697

Anti-GNPNAT1 antibody

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(1 Publication)

Rabbit Polyclonal GNPNAT1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GNPNAT1 aa 1 to C-terminus.

View Alternative Names

GNA1, GNPNAT1, Glucosamine 6-phosphate N-acetyltransferase, Phosphoglucosamine acetylase, Phosphoglucosamine transacetylase

1 Images
Western blot - Anti-GNPNAT1 antibody (AB127697)
  • WB

Unknown

Western blot - Anti-GNPNAT1 antibody (AB127697)

12% SDS PAGE

All lanes:

Western blot - Anti-GNPNAT1 antibody (ab127697) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human GNPNAT1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96EK6

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Alternative Products

Primary Antibodies

AB234981

Anti-GNPNAT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNPNAT1 antibody (AB234981)

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Complementary Products

Proteins & Peptides

AB101942

Recombinant Human GNPNAT1 protein

SDS-PAGE - Recombinant Human GNPNAT1 protein (AB101942)

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  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB2739

Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (AB2739)

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Primary Antibodies

AB203829

Anti-ZEB1 antibody [EPR17375]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] (AB203829)

  • 3
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNPNAT1 also known as Glucosamine-phosphate N-acetyltransferase plays a mechanical role as an enzyme in the biosynthesis of UDP-N-acetylglucosamine. This enzyme facilitates the transfer of an acetyl group from acetyl-CoA to glucosamine-6-phosphate forming N-acetylglucosamine-6-phosphate. With a molecular mass of approximately 26 kDa GNPNAT1 expresses in various tissues with significant levels detected in the liver and kidney among other organs.
Biological function summary

GNPNAT1 participates in the hexosamine biosynthetic pathway functioning in cellular processes like protein glycosylation. This protein does not act alone; it interacts with other enzymes to complete its function. GNPNAT1 activity influences the supply of N-acetyl-D-glucosamine an essential substrate for glycoprotein and glycolipid synthesis integral for proper cell signaling and structural firmness.

Pathways

GNPNAT1 plays a critical role in metabolic pathways particularly influencing the hexosamine biosynthetic pathway. This pathway connects to several metabolic networks including the production of glycosaminoglycans. It orchestrates with enzymes like glutamine fructose-6-phosphate amidotransferase (GFAT) to regulate the flux of substrates into these pathways therefore impacting energy metabolism and nutrient sensing.

GNPNAT1 implicates in conditions such as cancer and metabolic disorders. Abnormalities in its expression or function can lead to increased risks of various cancer types possibly due to disrupted glycosylation pathways affecting cell growth and adhesion. In diabetes the relationship between GNPNAT1 and GFAT might influence the development of insulin resistance highlighting its impact on glucose metabolism regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information GNPNAT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genome research 30:155-163 PubMed31953347

2020

A regulatory element modulates glioma cell sensitivity to temozolomide through long-range regulation of multiple target genes.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Anshun He,Bohan Chen,Jinfang Bi,Jun Chen,Dianhao Guo,Yuyang Qian,Wenbin Wang,Tengfei Shi,Zhongfang Zhao,Jiandang Shi,Woojin An,Frank Attenello,Wange Lu
View all publications
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Product promise

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