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AB234981

Anti-GNPNAT1 antibody

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(3 Publications)

Rabbit Polyclonal GNPNAT1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GNPNAT1.

View Alternative Names

GNA1, GNPNAT1, Glucosamine 6-phosphate N-acetyltransferase, Phosphoglucosamine acetylase, Phosphoglucosamine transacetylase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNPNAT1 antibody (AB234981)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNPNAT1 antibody (AB234981)

Paraffin-embedded human lung cancer tissue stained for GNPNAT1 with ab234981 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNPNAT1 antibody (AB234981)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNPNAT1 antibody (AB234981)

Paraffin-embedded human gastric cancer tissue stained for GNPNAT1 with ab234981 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-GNPNAT1 antibody (AB234981)
  • WB

Supplier Data

Western blot - Anti-GNPNAT1 antibody (AB234981)

All lanes:

Western blot - Anti-GNPNAT1 antibody (ab234981) at 1/500 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 2:

Mouse liver tissue lysate

Lane 3:

Mouse kidney tissue lysate

Lane 4:

Mouse stomach tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GNPNAT1.

Q96EK6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "500-5000 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNPNAT1 also known as Glucosamine-phosphate N-acetyltransferase plays a mechanical role as an enzyme in the biosynthesis of UDP-N-acetylglucosamine. This enzyme facilitates the transfer of an acetyl group from acetyl-CoA to glucosamine-6-phosphate forming N-acetylglucosamine-6-phosphate. With a molecular mass of approximately 26 kDa GNPNAT1 expresses in various tissues with significant levels detected in the liver and kidney among other organs.
Biological function summary

GNPNAT1 participates in the hexosamine biosynthetic pathway functioning in cellular processes like protein glycosylation. This protein does not act alone; it interacts with other enzymes to complete its function. GNPNAT1 activity influences the supply of N-acetyl-D-glucosamine an essential substrate for glycoprotein and glycolipid synthesis integral for proper cell signaling and structural firmness.

Pathways

GNPNAT1 plays a critical role in metabolic pathways particularly influencing the hexosamine biosynthetic pathway. This pathway connects to several metabolic networks including the production of glycosaminoglycans. It orchestrates with enzymes like glutamine fructose-6-phosphate amidotransferase (GFAT) to regulate the flux of substrates into these pathways therefore impacting energy metabolism and nutrient sensing.

GNPNAT1 implicates in conditions such as cancer and metabolic disorders. Abnormalities in its expression or function can lead to increased risks of various cancer types possibly due to disrupted glycosylation pathways affecting cell growth and adhesion. In diabetes the relationship between GNPNAT1 and GFAT might influence the development of insulin resistance highlighting its impact on glucose metabolism regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information GNPNAT1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2416590 PubMed40298908

2025

Nuclear-Localized BCKDK Facilitates Homologous Recombination Repair to Support Breast Cancer Progression and Therapy Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Haiying Liu,Jiaqian Feng,Tingting Pan,Pinggen Zhang,Ling Ye,Zetan Jiang,Zilong Zhou,Qiankun Mao,Jian Li,Xinyi Yang,Ping Gao,De Huang,Huafeng Zhang

Journal of clinical laboratory analysis 36:e24628 PubMed35929347

2022

Glucosamine-phosphate N-acetyltransferase 1 and its DNA methylation can be biomarkers for the diagnosis and prognosis of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Peikun Ding,Bin Peng,Guofeng Li,Xuefeng Sun,Guangsuo Wang

Journal of personalized medicine 11: PubMed34442440

2021

A Novel Approach for the Discovery of Biomarkers of Radiotherapy Response in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

James Meehan,Mark Gray,Carlos Martínez-Pérez,Charlene Kay,Jimi C Wills,Ian H Kunkler,J Michael Dixon,Arran K Turnbull
View all publications
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Product promise

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