Rabbit Recombinant Monoclonal GnRH antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat, Human, Transfected cell line - Human samples. Cited in 3 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (AB281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134634.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (AB281844)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (AB281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6583.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (AB281844)")
![Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] (AB281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-frozen-sections-img100603.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] (AB281844)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (AB281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123127.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (AB281844)")
![Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] (AB281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-frozen-sections-img18175.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] (AB281844)")
Publications
Filter by
- International journal of molecular sciences 24:2023CFTR Modulates Hypothalamic Neuron Excitability to Maintain Female Cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Wu,Yanting Que,Junjiang Chen,Lei Sun,Jinghui Guo,Ye Chun RuanPubMed 37628754
- Acta biochimica Polonica 70:183-1902023Timosaponin AIII attenuates precocious puberty in mice through downregulating the hypothalamic-pituitary-gonadal axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLili Zhou,Yaoquan Ren,Dongmei Li,Weiwei Zhou,Chengke Li,Qiang Wang,Xiangzheng YangPubMed 36928746
- Science advances 8:eabq29872022Hierarchical deployment of Tbx3 dictates the identity of hypothalamic KNDy neurons to control puberty onset.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Shi,Yanrong Zhuang,Zhenhua Chen,Mingrui Xu,Junqi Kuang,Xue-Lian Sun,Lisen Gao,Xia Kuang,Huairen Zhang,Wei Li,Samuel Zheng Hao Wong,Chuanyu Liu,Longqi Liu,Danhua Jiang,Duanqing Pei,Yi Lin,Qing-Feng WuPubMed 36383654
Key facts
- Isotype
- IgG1
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
| Transfected cell line - Human | Not recommended | Tested |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Species Rat | Dilution info 1/50 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/600 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/600 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line - Human | Dilution info 1/600 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.
Additional Targets
GnRH
Alternative names
GNRH, GRH, LHRH, GNRH1, Progonadoliberin-1, Progonadoliberin I
Recommended products
Rabbit Recombinant Monoclonal GnRH antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat, Human, Transfected cell line - Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG1
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24529-70
- Purification technique
- Affinity purification Protein A
- Specificity
Data from recombinant overexpression of GnRH demonstrating reactivity in WB, ICC, IP and Flow Cyt (intracelluar) is available.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gonadotropin-releasing hormone (GnRH) also referred to as luteinizing hormone-releasing hormone (LHRH) or gonadotropin peptide is a small peptide hormone with a molecular weight of approximately 1.2 kDa. GnRH is chiefly expressed in the hypothalamic neurons located in the pre-optic area of the brain. It is synthesized as a preprohormone and undergoes cleavage to become its active form. GnRH plays an important role in the regulation of reproductive functions acting as an important regulator of the release of pituitary gonadotropins.
- Biological function summary
GnRH influences reproduction by stimulating the anterior pituitary gland to secrete two essential gonadotropins: luteinizing hormone (LH) and follicle-stimulating hormone (FSH). These gonadotropins further act on the gonads to promote steroidogenesis and gametogenesis essential processes for fertility. The GnRH peptide hormone does not typically form part of a larger protein complex. Instead it interacts directly with specific receptors on the surface of anterior pituitary cells to carry out its functions.
- Pathways
The gonadotropin peptide acts within the hypothalamic-pituitary-gonadal (HPG) axis an integral pathway for maintaining reproductive health and hormonal balance. This pathway involves GnRH LH FSH and steroid hormones like estradiol and testosterone. In the HPG axis GnRH release is subjected to feedback mechanisms from the steroid hormones ensuring the fine-tuning of reproductive hormone levels. Additionally this peptide is part of the neuroendocrine control pathway interacting with other neuropeptides like kisspeptin which modulates its secretion thereby influencing overall reproductive function.
- Associated diseases and disorders
Dysfunctions in GnRH signaling can lead to conditions such as hypogonadotropic hypogonadism and precocious puberty. These disorders result from insufficient or excessive release of gonadotropins due to impaired GnRH action. In hypogonadotropic hypogonadism there is a failure in stimulation of LH and FSH leading to inadequate sex steroid production and subsequent infertility or delayed puberty. Conversely excessive GnRH activity may result in precocious puberty where early activation of the reproductive axis occurs. Anti-GnRH therapies aim to modulate these dysfunctions aligning related proteins like kisspeptin and neurokinin B with therapeutic approaches.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134634.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)
Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with human Progonadoliberin-1 expression vector containing a myc-His-tag® (Panel B) HEK-293T transfected with empty plasmid. Tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the arcuate hypothalamic nucleus arc of rat hypothalamus. The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6583.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)
Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the arcuate hypothalamic nucleus arc of mouse hypothalamus (PMID:19809474). The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-frozen-sections-img100603.jpg "Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hypothalamus tissue labelling GnRH with ab281844 at 150 (7.54 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on arcuate hypothalamic nucleus arc of mouse hypothalamus (arrow) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123127.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)
Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the arcuate hypothalamic nucleus arc of rat hypothalamus. The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-frozen-sections-img18175.jpg "Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hypothalamus tissue labelling GnRH with ab281844 at 150 (7.54 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on arcuate hypothalamic nucleus arc of rat hypothalamus (arrow) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79982.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human liver. The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98325.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse liver. The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29529.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat liver. The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79982.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98325.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844), expandable thumbnail](https://content.abcam.com/products/images/gnrh-antibody-epr24529-70-ab281844--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29529.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] (ab281844)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com