AB183079

Anti-GnRHR antibody

Name: Anti-GnRHR antibody (ab183079) | Abcam
Brand: Abcam Limited
SKU: AB183079
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(18 Publications)

Rabbit Polyclonal GNRHR antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human GNRHR aa 50-100.

View Alternative Names

GRHR, GNRHR, Gonadotropin-releasing hormone receptor, GnRH receptor, GnRH-R

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRHR antibody (AB183079)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRHR antibody (AB183079)

Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling GnRHR with ab183079 at 1/117 dilution. The right image is treated with the immunizing peptide.

Supplier Data

Western blot - Anti-GnRHR antibody (AB183079)

All lanes:

Western blot - Anti-GnRHR antibody (ab183079) at 1/2350 dilution

Lane 1:

231 cell lysate at 40 µg

Lane 2:

mouse testis tissue lysate at 40 µg

Secondary

All lanes:

Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

Predicted band size: 38 kDa

true

Exposure time: 25s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human GNRHR aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P30968

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/117 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GnRHR also known as the Gonadotropin-releasing hormone receptor is a G-protein coupled receptor that plays an important role in the reproductive system. This receptor has a molecular mass of approximately 37 kDa. GnRHR is mainly expressed in the pituitary gland but it is also found in other tissues like the ovary and prostate. Its primary function is to bind to the GnRH ligand which triggers the release of hormones such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH).

Biological function summary

The GnRHR is involved in regulating reproductive functions by mediating the action of GnRH at the pituitary level. It does not form part of larger complex structures like some other receptors might. This interaction leads to a second messenger cascade mainly involving phospholipase C which increases intracellular calcium levels. In turn hormone secretion is stimulated leading to gametogenesis and steroidogenesis.

Pathways

The GnRHR is integral to the hypothalamic-pituitary-gonadal (HPG) axis an important regulatory pathway in reproductive endocrinology. It works closely with proteins like FSH and LH which are essential for proper sexual development and reproductive health. The receptor activation initiates signaling pathways leading to the transcription of genes responsible for reproductive maturation and function.

GnRHR is notably associated with conditions like hypogonadotropic hypogonadism and polycystic ovary syndrome (PCOS). Mutations or malfunctions in this receptor or GnRH can lead to insufficient gonadotropin release. This malfunction disrupts the regular hormonal feedback loop necessary for reproduction. Furthermore altered signaling through GnRHR has connections to neoplastic disorders in tissues where it is expressed linking it to cancer pathways where proteins such as kisspeptin can also play a role.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 may act as an inhibitor of GnRH-R signaling.

See full target information GNRHR

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39451222

2024

Decreased Circulating Gonadotropin-Releasing Hormone Associated with Keratoconus.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Escandon,Alexander J Choi,Steve Mabry,Sarah E Nicholas,Rebecca L Cunningham,Liam Redden,David A Murphy,Kamran M Riaz,Tina B McKay,Dimitrios Karamichos

Pituitary 27:527-536 PubMed38954291

2024

Growth hormone increase by luteinizing hormone-releasing hormone reflects gonadotroph-related characteristics in acromegaly.

Applications

Unspecified application

Species

Unspecified reactive species

Yuto Mitsui,Kosuke Mukai,Michio Otsuki,Satoru Oshino,Youichi Saitoh,Masaharu Kohara,Eiichi Morii,Atsunori Fukuhara,Iichiro Shimomura

Molecular imaging 2023:6674054 PubMed38089464

2023

Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Peng,Qiyu Liu,Tao Pu,Mingxing Zhang,Meng Zhang,Ming Du,Guiling Li,Xiaoyan Zhang,Congjian Xu

International journal of molecular sciences 24: PubMed37686439

2023

Novel Correlation between TGF-β1/-β3 and Hormone Receptors in the Human Corneal Stroma.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander J Choi,Brenna S Hefley,Sarah E Nicholas,Rebecca L Cunningham,Dimitrios Karamichos

Acta biochimica Polonica 70:183-190 PubMed36928746

2023

Timosaponin AIII attenuates precocious puberty in mice through downregulating the hypothalamic-pituitary-gonadal axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zhou,Yaoquan Ren,Dongmei Li,Weiwei Zhou,Chengke Li,Qiang Wang,Xiangzheng Yang

The American journal of pathology 193:1762-1775 PubMed36822267

2023

Selective Modulation of the Keratoconic Stromal Microenvironment by FSH and LH.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Escandon,Sarah E Nicholas,Brenda Vasini,Rebecca L Cunningham,David A Murphy,Kamran M Riaz,Dimitrios Karamichos

Nutrition & metabolism 18:86 PubMed34530850

2021

Influence of HFD-induced precocious puberty on neurodevelopment in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tingbei Bo,Jing Wen,Wenting Gao,Liqiu Tang,Min Liu,Dehua Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:2000441 PubMed33643783

2021

Ultrabright NIR-II Emissive Polymer Dots for Metastatic Ovarian Cancer Detection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Zhou,Qiyu Liu,Wei Yuan,Zhenhua Li,Yuliang Xu,Wei Feng,Congjian Xu,Fuyou Li

Journal of gynecology obstetrics and human reprodu :101761 PubMed32325268

2020

Dysmenorrhea in patients with adenomyosis: A clinical and demographic study.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Jian Huang,Xu-Yin Zhang,Wei-Wei Feng,Ke-Qin Hua

Frontiers in oncology 10:266 PubMed32185134

2020

Gonadotropin-Releasing Hormone Receptor-Targeted Near-Infrared Fluorescence Probe for Specific Recognition and Localization of Peritoneal Metastases of Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyu Liu,Xiaobo Zhou,Wei Feng,Tao Pu,Xiaoping Li,Fuyou Li,Yu Kang,Xiaoyan Zhang,Congjian Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GnRHR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRHR antibody (AB183079)

Western blot - Anti-GnRHR antibody (AB183079)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

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