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AB325860

Anti-GNRHR antibody [HL2638] - BSA and Azide free

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Rabbit Recombinant Monoclonal GNRHR antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples. Immunogen corresponding to Synthetic Peptide within Human GNRHR.

View Alternative Names

GRHR, GNRHR, Gonadotropin-releasing hormone receptor, GnRH receptor, GnRH-R

3 Images
Western blot - Anti-GNRHR antibody [HL2638] - BSA and Azide free (AB325860)
  • WB

Supplier Data

Western blot - Anti-GNRHR antibody [HL2638] - BSA and Azide free (AB325860)

Boiled and unboiled PC-3 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325860 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE : whole cell extract; ME : membrane extract)

All lanes:

Western blot - Anti-GNRHR antibody [HL2638] - BSA and Azide free (ab325860) at 1/1000 dilution

Lane 1:

Boiled PC-3 whole cell extracts at 30 µg

Lane 2:

Boiled PC-3 membrane extracts at 30 µg

Lane 3:

Unboiled PC-3 whole cell extracts at 30 µg

Lane 4:

Unboiled PC-3 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GNRHR antibody [HL2638] - BSA and Azide free (AB325860)
  • WB

Supplier Data

Western blot - Anti-GNRHR antibody [HL2638] - BSA and Azide free (AB325860)

Non-transfected (–) and transfected (+) unboiled PC-3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325860 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GNRHR antibody [HL2638] - BSA and Azide free (ab325860) at 1/1000 dilution

Lane 1:

Non-transfected (–) unboiled PC-3 whole cell extracts at 30 µg

Lane 2:

GNRHR shRNA transfected (+) unboiled PC-3 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GNRHR antibody [HL2638] - BSA and Azide free (AB325860)
  • WB

Supplier Data

Western blot - Anti-GNRHR antibody [HL2638] - BSA and Azide free (AB325860)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325860 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GNRHR antibody [HL2638] - BSA and Azide free (ab325860) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

hFc-tagged GNRHR transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

hFc-tagged GNRHR transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2638

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GNRHR.

P30968

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 may act as an inhibitor of GnRH-R signaling.
See full target information GNRHR
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Product promise

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