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AB230044

Anti-GNS antibody

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(1 Publication)

Rabbit Polyclonal GNS antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human N-acetylglucosamine-6-sulfatase aa 250 to C-terminus.

View Alternative Names

N-acetylglucosamine-6-sulfatase, Glucosamine-6-sulfatase, G6S, GNS

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNS antibody (AB230044)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNS antibody (AB230044)

Paraffin-embedded human kidney tissue stained for GNS using ab230044 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNS antibody (AB230044)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNS antibody (AB230044)

Paraffin-embedded human testis tissue stained for GNS using ab230044 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-GNS antibody (AB230044)
  • WB

Supplier Data

Western blot - Anti-GNS antibody (AB230044)

All lanes:

Western blot - Anti-GNS antibody (ab230044) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 62 kDa

Observed band size: 27 kDa,37 kDa,62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human N-acetylglucosamine-6-sulfatase aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P15586

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GNS protein also known as glucosamine (N-acetyl)-6-sulfatase is an enzyme with significant roles in the breakdown of glycosaminoglycans. Its molecular weight is around 63 kDa. GNS is frequently expressed in lysosomal compartments where it catalyzes the cleavage of sulfate groups from N-acetylglucosamine residues in heparan sulfate and other similar molecules. This process is critical for the degradation and recycling of complex carbohydrates in cells.
Biological function summary

Glucosamine (N-acetyl)-6-sulfatase activity influences cellular homeostasis by acting within the lysosomal hydrolase family. GNS participates in the breakdown and metabolism of glycosaminoglycans important for maintaining cellular integrity and signaling functions. It operates as a standalone enzyme rather than part of a larger complex. Through its enzymatic function GNS helps prevent the accumulation of partially degraded molecules inside lysosomes.

Pathways

Glucosamine (N-acetyl)-6-sulfatase activity plays a role in lysosomal storage pathways and in the catabolic processes of heparan sulfate. This enzyme works alongside other lysosomal proteins like iduronate sulfatase and alpha-L-iduronidase which are also involved in glycosaminoglycan degradation pathways. Proper function of these pathways is important for cellular waste management and nutrient recycling.

Altered GNS function relates to conditions such as mucopolysaccharidosis type IIID (Sanfilippo syndrome type D) and some forms of lysosomal storage diseases. Deficiencies in GNS disrupt normal lysosomal breakdown of glycosaminoglycans leading to accumulations that can cause cellular damage. This disorder often involves other metabolic proteins like heparan N-sulfatase and alpha-N-acetylglucosaminidase. Understanding the role of GNS offers insights into potential therapeutic approaches for related metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information N-acetylglucosamine-6-sulfatase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:944591 PubMed36091013

2022

Multi-omics analysis reveals the metabolic regulators of duodenal low-grade inflammation in a functional dyspepsia model.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Ji,Yanting You,Baizhao Peng,Tianyu Zhong,Yuxiang Kuang,Shasha Li,Lijing Du,Liqian Chen,Xiaomin Sun,Jiaojiao Dai,Suiping Huang,Yuyao Wu,Yanyan Liu
View all publications
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