AB80589

Anti-GAD67/GAD1 antibody

Name: Anti-GAD67/GAD1 antibody (ab80589) | Abcam
Brand: Abcam Limited
SKU: AB80589
Price: 535 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(6 Publications)

Goat Polyclonal GAD67 antibody. Suitable for WB, IHC-P, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human GAD1 aa 500-550.

View Alternative Names

GAD, GAD67, GAD1, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD-67

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody (AB80589)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody (AB80589)

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry - Anti-GAD67/GAD1 antibody (AB80589)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-GAD67/GAD1 antibody (AB80589)

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Unknown

Western blot - Anti-GAD67/GAD1 antibody (AB80589)

All lanes:

Western blot - Anti-GAD67/GAD1 antibody (ab80589) at 0.5 µg/mL

All lanes:

Human brain (cerebral cortex) lysate in RIPA buffer at 35 µg

Predicted band size: 66 kDa

Observed band size: 67 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human GAD1 aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

Q99259

Specificity

ab80589 is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2 (isoform GAD65).

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab80589 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor.. Isoform 3. Enzymatically inactive as glutamate decarboxylase.

See full target information GAD1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular pain 16:1744806920971377 PubMed33297861

2020

Plastic changes in amygdala subregions by voluntary running contribute to exercise-induced hypoalgesia in neuropathic pain model mice.

Applications

Unspecified application

Species

Unspecified reactive species

Katsuya Kami,Fumihiro Tajima,Emiko Senba

Stem cell research 49:102065 PubMed33137567

2020

Methadone interrupts neural growth and function in human cortical organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Yao,Wei Wu,Ines Cerf,Helen W Zhao,Juan Wang,Priscilla D Negraes,Alysson R Muotri,Gabriel G Haddad

The Journal of comparative neurology 527:2843-2859 PubMed31050805

2019

Ferret brain possesses young interneuron collections equivalent to human postnatal migratory streams.

Applications

Unspecified application

Species

Unspecified reactive species

Justin K Ellis,Shawn F Sorrells,Sasha Mikhailova,Manideep Chavali,Sandra Chang,Khalida Sabeur,Patrick Mcquillen,David H Rowitch

Journal of neurovirology 25:520-524 PubMed31025264

2019

JC virus infection of meningeal and choroid plexus cells in patients with progressive multifocal leukoencephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah M Corbridge,Richard C Rice,Linda A Bean,Christian Wüthrich,Xin Dang,Daniel A Nicholson,Igor J Koralnik

The Journal of clinical investigation 128:5413-5427 PubMed30226827

2018

Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Qian-Xing Zhuang,Guang-Ying Li,Bin Li,Chang-Zheng Zhang,Xiao-Yang Zhang,Kang Xi,Hong-Zhao Li,Jian-Jun Wang,Jing-Ning Zhu

The Journal of comparative neurology 522:3308-34 PubMed24715505

2014

GABAergic and glutamatergic efferents of the mouse ventral tegmental area.

Applications

IHC-FrFl

Species

Mouse

Seth R Taylor,Sylvia Badurek,Ralph J Dileone,Raad Nashmi,Liliana Minichiello,Marina R Picciotto

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAD67/GAD1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody (AB80589)

Flow Cytometry - Anti-GAD67/GAD1 antibody (AB80589)

Western blot - Anti-GAD67/GAD1 antibody (AB80589)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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