Goat Polyclonal GAD67 antibody. Suitable for WB, IHC-P, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human GAD1 aa 500-550.
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Publications
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- Stem cell research 49:1020652020Methadone interrupts neural growth and function in human cortical organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHang Yao et. al.PubMed 33137567
- The Journal of comparative neurology 527:2843-28592019Ferret brain possesses young interneuron collections equivalent to human postnatal migratory streams.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJustin K Ellis et. al.PubMed 31050805
- Journal of neurovirology 25:520-5242019JC virus infection of meningeal and choroid plexus cells in patients with progressive multifocal leukoencephalopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah M Corbridge et. al.PubMed 31025264
- The Journal of clinical investigation 128:5413-54272018Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian-Xing Zhuang,Guang-Ying Li,Bin Li,Chang-Zheng Zhang,Xiao-Yang Zhang,Kang Xi,Hong-Zhao Li,Jian-Jun Wang,Jing-Ning ZhuPubMed 30226827
- The Journal of comparative neurology 522:3308-342014GABAergic and glutamatergic efferents of the mouse ventral tegmental area.Applications:IHC-FrFlReactive species:MouseSeth R Taylor,Sylvia Badurek,Ralph J Dileone,Raad Nashmi,Liliana Minichiello,Marina R PicciottoPubMed 24715505
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GAD1 aa 500-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q99259
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Reactivity data
Select an application| WB | IHC-P | Flow Cyt | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Cat | Predicted | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1.5 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Chimpanzee, Orangutan | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Chimpanzee, Orangutan | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Chimpanzee, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Chimpanzee, Orangutan | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor. Isoform 3. Enzymatically inactive as glutamate decarboxylase.
Alternative names
GAD, GAD67, GAD1, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD-67
Recommended products
Goat Polyclonal GAD67 antibody. Suitable for WB, IHC-P, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human GAD1 aa 500-550.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GAD1 aa 500-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q99259
- Purification technique
- Affinity purification Immunogen
- Specificity
ab80589 is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2 (isoform GAD65).
- Concentration
- Purification notes
ab80589 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Goat polyclonal to GAD67/GAD1 (ab80589)
All lanes: Western blot - Goat polyclonal to GAD67/GAD1 (ab80589) at 0.5 µg/mL
All lanes: Human brain (cerebral cortex) lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Observed band size: 67 kDa
Immunocytochemistry/ Immunofluorescence - Goat polyclonal to GAD67/GAD1 (ab80589)
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry - Goat polyclonal to GAD67/GAD1 (ab80589)
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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