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AB155806

Anti-GOLGA5/Golgin-84 antibody

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(1 Publication)

Rabbit Polyclonal GOLGA5/Golgin-84 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GOLGA5 aa 200-600.

View Alternative Names

RETII, RFG5, PIG31, GOLGA5, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein, Golgin-84, Protein Ret-II, RET-fused gene 5 protein

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (AB155806)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (AB155806)

Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling GOLGA5/Golgin-84 with ab155806 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an anti-alpha-tubulin antibody at 1/2500 (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB155806)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB155806)

Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue, labeling GOLGA5/Golgin-84 with ab155806 at 1/100 dilution.

Western blot - Anti-GOLGA5/Golgin-84 antibody (AB155806)
  • WB

Unknown

Western blot - Anti-GOLGA5/Golgin-84 antibody (AB155806)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GOLGA5/Golgin-84 antibody (ab155806) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 83 kDa

false

Western blot - Anti-GOLGA5/Golgin-84 antibody (AB155806)
  • WB

Unknown

Western blot - Anti-GOLGA5/Golgin-84 antibody (AB155806)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GOLGA5/Golgin-84 antibody (ab155806) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GOLGA5 aa 200-600. The exact immunogen used to generate this antibody is proprietary information.

Q8TBA6

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GOLGA5 also known as Golgin-84 is an important player in maintaining the structural integrity of the Golgi apparatus. This protein weighs approximately 84 kDa and exists within the Golgi membrane structure. It facilitates the tethering and fusion of vesicles ensuring the proper flow of proteins and lipids through the Golgi network. Golgin-84 expresses in a variety of tissues with strong local presence in cells undergoing secretion indicating its role in cellular transport processes.
Biological function summary

GOLGA5 participates in the assembly of the Golgi ribbon a structure essential for the efficient functioning of the Golgi apparatus. This protein integrates into a multiprotein complex which is critical for maintaining the stacked and ribbon-like structure of the Golgi. Its interaction with other Golgi matrix proteins helps regulate the positioning and coherence of Golgi cisternae. This ensures proper vesicle transfer and contributes to overall cellular homeostasis.

Pathways

The function of GOLGA5 is critical in the secretory pathway where it ensures the orderly transport of proteins processed by the Golgi apparatus. The presence of GOLGA5 supports Golgi reassembly and stacking protein 65 (GRASP65) involvement in Golgi reformation during the cell cycle. Its function also connects with ER-to-Golgi transport helping maintain an important support system for intracellular trafficking.

GOLGA5 appears related to neurodegenerative diseases and cancer. Alterations in its function or expression could dispose cells to develop cellular transport disruptions potentially leading to illnesses like Alzheimer’s disease where protein transport and processing are compromised. Furthermore in cases of cancer such as prostate cancer changes in GOLGA5 expression might disrupt normal cell function linking it to pathways involving oncoproteins.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.
See full target information GOLGA5

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:438-448 PubMed39984653

2025

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties.

Applications

Unspecified application

Species

Unspecified reactive species

Dennis K Jeppesen,Zachary C Sanchez,Noah M Kelley,James B Hayes,Jessica Ambroise,Emma N Koory,Evan Krystofiak,Nilay Taneja,Qin Zhang,Matthew M Dungan,Olivia L Perkins,Matthew J Tyska,Ela W Knapik,Kevin M Dean,Amanda C Doran,Robert J Coffey,Dylan T Burnette
View all publications

Product promise

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