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Rabbit Polyclonal GOLGA5/Golgin-84 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Golgin subfamily A member 5 aa 200-600.

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Images

Western blot - Anti-GOLGA5/Golgin-84 antibody (AB155806), expandable thumbnail
  • Western blot - Anti-GOLGA5/Golgin-84 antibody (AB155806), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (AB155806), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB155806), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Golgin subfamily A member 5 aa 200-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TBA6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.

Alternative names

Recommended products

Rabbit Polyclonal GOLGA5/Golgin-84 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Golgin subfamily A member 5 aa 200-600.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Golgin subfamily A member 5 aa 200-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TBA6
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GOLGA5 also known as Golgin-84 is an important player in maintaining the structural integrity of the Golgi apparatus. This protein weighs approximately 84 kDa and exists within the Golgi membrane structure. It facilitates the tethering and fusion of vesicles ensuring the proper flow of proteins and lipids through the Golgi network. Golgin-84 expresses in a variety of tissues with strong local presence in cells undergoing secretion indicating its role in cellular transport processes.

Biological function summary

GOLGA5 participates in the assembly of the Golgi ribbon a structure essential for the efficient functioning of the Golgi apparatus. This protein integrates into a multiprotein complex which is critical for maintaining the stacked and ribbon-like structure of the Golgi. Its interaction with other Golgi matrix proteins helps regulate the positioning and coherence of Golgi cisternae. This ensures proper vesicle transfer and contributes to overall cellular homeostasis.

Pathways

The function of GOLGA5 is critical in the secretory pathway where it ensures the orderly transport of proteins processed by the Golgi apparatus. The presence of GOLGA5 supports Golgi reassembly and stacking protein 65 (GRASP65) involvement in Golgi reformation during the cell cycle. Its function also connects with ER-to-Golgi transport helping maintain an important support system for intracellular trafficking.

Associated diseases and disorders

GOLGA5 appears related to neurodegenerative diseases and cancer. Alterations in its function or expression could dispose cells to develop cellular transport disruptions potentially leading to illnesses like Alzheimer’s disease where protein transport and processing are compromised. Furthermore in cases of cancer such as prostate cancer changes in GOLGA5 expression might disrupt normal cell function linking it to pathways involving oncoproteins.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-GOLGA5/Golgin-84 antibody (ab155806), expandable thumbnail

    Western blot - Anti-GOLGA5/Golgin-84 antibody (ab155806)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-GOLGA5/Golgin-84 antibody (ab155806) at 1/1000 dilution

    All lanes: A549 whole cell lysate at 30 µg

    Predicted band size: 83 kDa

  • Western blot - Anti-GOLGA5/Golgin-84 antibody (ab155806), expandable thumbnail

    Western blot - Anti-GOLGA5/Golgin-84 antibody (ab155806)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-GOLGA5/Golgin-84 antibody (ab155806) at 1/1000 dilution

    All lanes: Mouse brain whole cell lysate at 50 µg

    Predicted band size: 83 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (ab155806), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (ab155806)

    Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling GOLGA5/Golgin-84 with ab155806 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an anti-alpha-tubulin antibody at 1/2500 (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (ab155806), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (ab155806)

    Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue, labeling GOLGA5/Golgin-84 with ab155806 at 1/100 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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