AB224040

Anti-GOLGA5/Golgin-84 antibody

Name: Anti-GOLGA5/Golgin-84 antibody (ab224040) | Abcam
Brand: Abcam Limited
SKU: AB224040
Price: 645 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GOLGA5/Golgin-84 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GOLGA5 aa 150-350.

View Alternative Names

RETII, RFG5, PIG31, GOLGA5, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein, Golgin-84, Protein Ret-II, RET-fused gene 5 protein

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human epididymis tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (AB224040)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (AB224040)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for GOLGA5/Golgin-84 (green) using ab224040 at 4 μg/ml in ICC/IF.

Supplier Data

Western blot - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Loading control : GAPDH

All lanes:

Western blot - Anti-GOLGA5/Golgin-84 antibody (ab224040) at 0.4 µg/mL

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells transfected with control siRNA, whole cell lysate

Lane 2:

U-87MG cells transfected with target specific siRNA probe #1, whole cell lysate

Lane 3:

U-87MG cells transfected with target specific siRNA probe #2, whole cell lysate

Predicted band size: 83 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GOLGA5 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q8TBA6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200.00000 - 1/500.00000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04000-0.40000 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25000-2.00000 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GOLGA5 also known as Golgin-84 is an important player in maintaining the structural integrity of the Golgi apparatus. This protein weighs approximately 84 kDa and exists within the Golgi membrane structure. It facilitates the tethering and fusion of vesicles ensuring the proper flow of proteins and lipids through the Golgi network. Golgin-84 expresses in a variety of tissues with strong local presence in cells undergoing secretion indicating its role in cellular transport processes.

Biological function summary

GOLGA5 participates in the assembly of the Golgi ribbon a structure essential for the efficient functioning of the Golgi apparatus. This protein integrates into a multiprotein complex which is critical for maintaining the stacked and ribbon-like structure of the Golgi. Its interaction with other Golgi matrix proteins helps regulate the positioning and coherence of Golgi cisternae. This ensures proper vesicle transfer and contributes to overall cellular homeostasis.

Pathways

The function of GOLGA5 is critical in the secretory pathway where it ensures the orderly transport of proteins processed by the Golgi apparatus. The presence of GOLGA5 supports Golgi reassembly and stacking protein 65 (GRASP65) involvement in Golgi reformation during the cell cycle. Its function also connects with ER-to-Golgi transport helping maintain an important support system for intracellular trafficking.

GOLGA5 appears related to neurodegenerative diseases and cancer. Alterations in its function or expression could dispose cells to develop cellular transport disruptions potentially leading to illnesses like Alzheimer’s disease where protein transport and processing are compromised. Furthermore in cases of cancer such as prostate cancer changes in GOLGA5 expression might disrupt normal cell function linking it to pathways involving oncoproteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.

See full target information GOLGA5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroscience methods 361:109285 PubMed34242703

2021

Protein-retention expansion microscopy for visualizing subcellular organelles in fixed brain tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Logan A Campbell,Katy E Pannoni,Niesha A Savory,Dinesh Lal,Shannon Farris

Frontiers in cellular and infection microbiology 11:675890 PubMed34169005

2021

Akt Phosphorylation Influences Persistent Chlamydial Infection and -Induced Golgi Fragmentation Without Involving Rab14.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobao Huang,Jinfeng Tan,Xiaohong Chen,Mingna Liu,Huiling Zhu,Wenjing Li,Zhenjian He,Jiande Han,Chunguang Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Associated Products

Select an associated product type

Alternative Product

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golga5-golgin-84-antibody-ab155806

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