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AB122250

Anti-Golgin-245 antibody

5

(5 Reviews)

|

(2 Publications)

Rabbit Polyclonal Golgin-245 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GOLGA4 aa 1450-1600.

View Alternative Names

Golgin subfamily A member 4, 256 kDa golgin, Golgin-245, Protein 72.1, Trans-Golgi p230, GOLGA4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Golgin-245 antibody (AB122250)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Golgin-245 antibody (AB122250)

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & vesicles.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Golgin-245 antibody (AB122250)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Golgin-245 antibody (AB122250)

ab122250, at 1/1200, staining Golgin-245 in paraffin embedded Human stomach tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GOLGA4 aa 1450-1600. The exact immunogen used to generate this antibody is proprietary information.

Q13439

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Golgin-245 also known as GM130L or Golgin subfamily A member 4 is an important component in membrane trafficking processes involving the Golgi apparatus. This protein has an approximate mass of 245 kDa and is expressed mainly in the perinuclear region of mammalian cells. It plays a role in maintaining the structural integrity of the Golgi by facilitating vesicle tethering which is important for the movement of proteins and lipids through the secretory pathway.
Biological function summary

Golgin-245 interacts with other proteins to ensure proper Golgi function and organization. It is part of a larger protein complex that participates in vesicle docking and fusion events. By anchoring transport vesicles Golgin-245 ensures that cargo is accurately delivered supporting cell-secretory processes. This interaction enables Golgin-245 to contribute to the maintenance of efficient intracellular communication and protein processing ultimately impacting various physiological processes.

Pathways

Golgin-245 plays a critical role in the secretory pathway. This pathway involves the transport of proteins from the endoplasmic reticulum through the Golgi apparatus to their final destinations. Golgin-245 works alongside proteins like Rab6 and Arf1 which regulate vesicle formation and movement. These proteins and Golgin-245 coordinate to ensure the smooth transit of vesicles highlighting Golgin-245's important function in maintaining cellular homeostasis.

Golgin-245 has been implicated in conditions like neurodegenerative diseases and certain types of cancer. Its function of mediating proper protein transport is linked with proteins such as β-amyloid precursor protein in Alzheimer's disease. Aberrations in Golgin-245's activity can lead to disruptions in protein trafficking which has been observed in neurodegeneration. Furthermore its altered expression is associated with cancer progression where improper protein distribution contributes to abnormal cell proliferation and tumor growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in vesicular trafficking at the Golgi apparatus level. May play a role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with MACF1. Involved in endosome-to-Golgi trafficking (PubMed : 29084197).
See full target information GOLGA4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:1537-1558 PubMed38591519

2024

Alcohol-induced Golgiphagy is triggered by the downregulation of Golgi GTPase RAB3D.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda J Macke,Taylor E Divita,Artem N Pachikov,Sundararajan Mahalingam,Ramesh Bellamkonda,Karuna Rasineni,Carol A Casey,Armen Petrosyan

Nucleic acids research 50:10503-10525 PubMed36200829

2022

APE1 assembles biomolecular condensates to promote the ATR-Chk1 DNA damage response in nucleolus.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Li,Haichao Zhao,Anne McMahon,Shan Yan
View all publications
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Product promise

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