AB169287

Anti-Golgin 97 antibody

Name: Anti-Golgin 97 antibody (ab169287) | Abcam
Brand: Abcam Limited
SKU: AB169287
Price: 545 USD
Availability: InStock

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(4 Publications)

Mouse Polyclonal Golgin 97 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GOLGA1.

View Alternative Names

Golgin subfamily A member 1, Golgin-97, GOLGA1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Golgin 97 antibody (AB169287)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Golgin 97 antibody (AB169287)

Immunofluorescent analysis of HeLa cells labeling Golgin 97 with ab169287 at 10 μg/ml.

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Western blot - Anti-Golgin 97 antibody (AB169287)

All lanes:

Western blot - Anti-Golgin 97 antibody (ab169287) at 1 µg/mL

All lanes:

Human pancreas tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 88 kDa

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Western blot - Anti-Golgin 97 antibody (AB169287)

All lanes:

Western blot - Anti-Golgin 97 antibody (ab169287) at 1 µg/mL

Lane 1:

Golgin 97 transfected 293T lysate at 15 µL

Lane 2:

Non-transfected 293T lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 88 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GOLGA1.

Q92805

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Golgin-97 also known as Golgin subfamily A member 1 is a protein that plays a significant role in the Golgi apparatus function. This protein has a molecular mass of approximately 97 kDa. Golgin-97 is widely expressed in the Golgi apparatus of eukaryotic cells. Its structure facilitates its function as a membrane-bound protein influencing the organization of the Golgi stack and maintenance of the Golgi ribbon.

Biological function summary

Golgin-97 participates in tethering of transport vesicles to the Golgi membrane ensuring efficient trafficking of proteins to their correct destinations. It is part of the golgin family which includes several coiled-coil proteins critical for Golgi structure and dynamics. Golgin-97 acts within a larger protein complex that interacts with other Golgi-associated proteins to regulate vesicle docking and fusion.

Pathways

Protein and lipid trafficking involve Golgin-97 significantly as it aids in vesicular transport coordination from the endoplasmic reticulum to the Golgi and within the Golgi stacks. The protein interacts closely with Rab GTPases a class of proteins that regulate vesicle movement. Golgin-97's function intertwines with pathways like the secretory pathway and the endocytic pathway linking it to other proteins such as Rab6 and Rab9.

Golgin-97 has associations with pathologies involving Golgi dysfunction and transport defects. For instance it relates to diseases like neurodegenerative disorders where proper protein trafficking is disrupted. The malfunction of Golgin-97 can affect proteins like Alzheimer's disease proteins as vesicular transport failure can lead to their mislocalization or aggregation contributing to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Involved in vesicular trafficking at the Golgi apparatus level. Involved in endosome-to-Golgi trafficking.

See full target information GOLGA1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Archives of biochemistry and biophysics 758:110049 PubMed38879142

2024

The lipid flippase ATP8A1 regulates the recruitment of ARF effectors to the trans-Golgi Network.

Applications

Unspecified application

Species

Unspecified reactive species

Cristian A Pocognoni,Tomasz Nawara,Jay M Bhatt,Eunjoo Lee,Xiaoying Jian,Paul Randazzo,Elizabeth Sztul

Nature communications 13:1058 PubMed35217685

2022

AP-4-mediated axonal transport controls endocannabinoid production in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra K Davies,Julian E Alecu,Marvin Ziegler,Catherine G Vasilopoulou,Fabrizio Merciai,Hellen Jumo,Wardiya Afshar-Saber,Mustafa Sahin,Darius Ebrahimi-Fakhari,Georg H H Borner

Nature genetics 50:1021-1031 PubMed29892014

2018

Gorab is a Golgi protein required for structure and duplication of Drosophila centrioles.

Applications

Unspecified application

Species

Unspecified reactive species

Levente Kovacs,Jennifer Chao-Chu,Sandra Schneider,Marco Gottardo,George Tzolovsky,Nikola S Dzhindzhev,Maria Giovanna Riparbelli,Giuliano Callaini,David M Glover

Biochemical pharmacology 122:42-61 PubMed27816546

2016

Anhydroicaritin improves diet-induced obesity and hyperlipidemia and alleviates insulin resistance by suppressing SREBPs activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zu-Guo Zheng,Ya-Ping Zhou,Xin Zhang,Pyone Myat Thu,Zhi-Shen Xie,Chong Lu,Tao Pang,Bin Xue,Da-Qian Xu,Yan Chen,Xiao-Wei Chen,Hui-Jun Li,Xiaojun Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Golgin 97 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Golgin 97 antibody (AB169287)

Western blot - Anti-Golgin 97 antibody (AB169287)

Western blot - Anti-Golgin 97 antibody (AB169287)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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