AB228919

Anti-Golgin 97 antibody - C-terminal

Name: Anti-Golgin 97 antibody - C-terminal (ab228919) | Abcam
Brand: Abcam Limited
SKU: AB228919

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal Golgin 97 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GOLGA1.

View Alternative Names

Golgin subfamily A member 1, Golgin-97, GOLGA1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Golgin 97 antibody - C-terminal (AB228919)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Golgin 97 antibody - C-terminal (AB228919)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Golgin 97 protein using ab228919 at a 1/500 dilution (Green).
alpha Tubulin antibody was used at 1/1000 dilution (Red)
DAPI was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-Golgin 97 antibody - C-terminal (AB228919)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Golgin 97 antibody - C-terminal (ab228919) at 1/1000 dilution

Lane 1:

BT-474 whole cell extracts at 30 µg

Lane 2:

Ramos whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GOLGA1. The exact immunogen used to generate this antibody is proprietary information.

Q92805

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Golgin-97 also known as Golgin subfamily A member 1 is a protein that plays a significant role in the Golgi apparatus function. This protein has a molecular mass of approximately 97 kDa. Golgin-97 is widely expressed in the Golgi apparatus of eukaryotic cells. Its structure facilitates its function as a membrane-bound protein influencing the organization of the Golgi stack and maintenance of the Golgi ribbon.

Biological function summary

Golgin-97 participates in tethering of transport vesicles to the Golgi membrane ensuring efficient trafficking of proteins to their correct destinations. It is part of the golgin family which includes several coiled-coil proteins critical for Golgi structure and dynamics. Golgin-97 acts within a larger protein complex that interacts with other Golgi-associated proteins to regulate vesicle docking and fusion.

Pathways

Protein and lipid trafficking involve Golgin-97 significantly as it aids in vesicular transport coordination from the endoplasmic reticulum to the Golgi and within the Golgi stacks. The protein interacts closely with Rab GTPases a class of proteins that regulate vesicle movement. Golgin-97's function intertwines with pathways like the secretory pathway and the endocytic pathway linking it to other proteins such as Rab6 and Rab9.

Golgin-97 has associations with pathologies involving Golgi dysfunction and transport defects. For instance it relates to diseases like neurodegenerative disorders where proper protein trafficking is disrupted. The malfunction of Golgin-97 can affect proteins like Alzheimer's disease proteins as vesicular transport failure can lead to their mislocalization or aggregation contributing to disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in vesicular trafficking at the Golgi apparatus level. Involved in endosome-to-Golgi trafficking. Mechanistically, captures transport vesicles arriving from endosomes via the protein TBC1D23 (PubMed : 29084197, PubMed : 38552021). Recognized vesicles are then tethered to the trans-Golgi before subsequent SNARE engagement and vesicle fusion. Selectively regulates E-cadherin transport from the trans-Golgi network in tubulovesicular carriers (PubMed : 34969853).. (Microbial infection) Plays an important role in poxvirus morphogenesis. Translocates into the viral factories where it may transport the membrane fragments and associated protein factors important for virus maturation to the sites of virion assembly.

See full target information GOLGA1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38474091

2024

UBA6 Inhibition Accelerates Lysosomal TRPML1 Depletion and Exosomal Secretion in Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dongun Lee,Peter Chang-Whan Lee,Jeong Hee Hong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Golgin 97 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-Golgin 97 antibody - C-terminal (AB228919)

Western blot - Anti-Golgin 97 antibody - C-terminal (AB228919)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise