Anti-Golgin 97 antibody [EPR13806]

• WB

Supplier Data

Western blot - Anti-Golgin 97 antibody [EPR13806] (AB180935)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Golgin 97 antibody [EPR13806] (ab180935) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

SW480 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 88 kDa

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• WB

Lab

Western blot - Anti-Golgin 97 antibody [EPR13806] (AB180935)

Western blot : Anti-GOLGA1 antibody [EPR13806] (ab180935) staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab180935 was shown to bind specifically to GOLGA1. A band was observed at 99 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in GOLGA1 knockout cell line. To generate this image, wild-type and GOLGA1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Golgin 97 antibody [EPR13806] (ab180935) at 1/10000 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

GOLGA1 knockout HCT 116 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Ramos cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 99 kDa

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