Skip to main content

Knockout Tested Rabbit Recombinant Monoclonal Golgin 97 antibody. Suitable for WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Golgin 97 antibody [EPR13806] (AB180935), expandable thumbnail
  • Western blot - Anti-Golgin 97 antibody [EPR13806] (AB180935), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Involved in vesicular trafficking at the Golgi apparatus level. Involved in endosome-to-Golgi trafficking.

Alternative names

Golgin subfamily A member 1, Golgin-97, GOLGA1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Golgin 97 antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13806
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Golgin-97 also known as Golgin subfamily A member 1 is a protein that plays a significant role in the Golgi apparatus function. This protein has a molecular mass of approximately 97 kDa. Golgin-97 is widely expressed in the Golgi apparatus of eukaryotic cells. Its structure facilitates its function as a membrane-bound protein influencing the organization of the Golgi stack and maintenance of the Golgi ribbon.

Biological function summary

Golgin-97 participates in tethering of transport vesicles to the Golgi membrane ensuring efficient trafficking of proteins to their correct destinations. It is part of the golgin family which includes several coiled-coil proteins critical for Golgi structure and dynamics. Golgin-97 acts within a larger protein complex that interacts with other Golgi-associated proteins to regulate vesicle docking and fusion.

Pathways

Protein and lipid trafficking involve Golgin-97 significantly as it aids in vesicular transport coordination from the endoplasmic reticulum to the Golgi and within the Golgi stacks. The protein interacts closely with Rab GTPases a class of proteins that regulate vesicle movement. Golgin-97's function intertwines with pathways like the secretory pathway and the endocytic pathway linking it to other proteins such as Rab6 and Rab9.

Associated diseases and disorders

Golgin-97 has associations with pathologies involving Golgi dysfunction and transport defects. For instance it relates to diseases like neurodegenerative disorders where proper protein trafficking is disrupted. The malfunction of Golgin-97 can affect proteins like Alzheimer's disease proteins as vesicular transport failure can lead to their mislocalization or aggregation contributing to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Golgin 97 antibody [EPR13806] (ab180935), expandable thumbnail

    Western blot - Anti-Golgin 97 antibody [EPR13806] (ab180935)

    Blocking/ Dilution buffer: 5% NFDM /TBST.

    All lanes: Western blot - Anti-Golgin 97 antibody [EPR13806] (ab180935) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: SW480 cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 88 kDa

  • Western blot - Anti-Golgin 97 antibody [EPR13806] (ab180935), expandable thumbnail

    Western blot - Anti-Golgin 97 antibody [EPR13806] (ab180935)

    Western blot: Anti-GOLGA1 antibody [EPR13806] (ab180935) staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab180935 was shown to bind specifically to GOLGA1. A band was observed at 99 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in GOLGA1 knockout cell line. To generate this image, wild-type and GOLGA1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Golgin 97 antibody [EPR13806] (ab180935) at 1/10000 dilution

    Lane 1: Wild-type HCT 116 cell lysate at 20 µg

    Lane 2: GOLGA1 knockout HCT 116 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: Ramos cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 99 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com