AB236296

Anti-GOLPH3/MIDAS antibody

Name: Anti-GOLPH3/MIDAS antibody (ab236296) | Abcam
Brand: Abcam Limited
SKU: AB236296
Price: 525 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal GOLPH3/MIDAS antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GOLPH3 aa 1-150.

View Alternative Names

GPP34, GOLPH3, Golgi phosphoprotein 3, Coat protein GPP34, Mitochondrial DNA absence factor, MIDAS

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (AB236296)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (AB236296)

Immunohistochemical analysis of formalin fixed paraffin embedded human prostate cancer tissue labelling GOLPH3/MIDAS with ab236296 at a 1 : 1200 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab236296 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

Supplier Data

Immunoprecipitation - Anti-GOLPH3/MIDAS antibody (AB236296)

Immunoprecipitation of GOLPH3 in Hela whole cell lysate. Lane 1 : Rabbit control IgG instead of ab236296 in Hela whole cell lysate Lane 2 : ab236296 (7.98µg)+ Hela whole cell lysate (600µg) Lane 3 : Hela whole cell lysate (30µg) For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/20000)

All lanes:

Immunoprecipitation - Anti-GOLPH3/MIDAS antibody (ab236296)

All lanes:

Hela whole cell lysate

Predicted band size: 34 kDa

Observed band size: 34 kDa

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Supplier Data

Western blot - Anti-GOLPH3/MIDAS antibody (AB236296)

All lanes:

Western blot - Anti-GOLPH3/MIDAS antibody (ab236296) at 1/1000 dilution

Lane 1:

Hela whole cell lysate at 20 µg

Lane 2:

MCF7 whole cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human GOLPH3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9H4A6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GOLPH3 also known as MIDAS (Mitochondrial Dynamics Associated) is a protein with a molecular weight of approximately 34 kDa. It is highly expressed in the Golgi apparatus a critical organelle for processing and sorting cellular proteins. GOLPH3 interacts with other proteins and lipids within the Golgi facilitating vesicle budding which is necessary for protein trafficking and secretion. The structural features of GOLPH3 allow it to bind phosphoinositides which indicates its role as a phosphatidylinositol 4-phosphate effector in the Golgi.

Biological function summary

GOLPH3/MIDAS plays a central role in maintaining Golgi structure and functionality. Its involvement in protein trafficking stems from its interaction with the cytoskeleton and Golgi membranes. GOLPH3 is part of a complex that includes several other proteins enhancing its ability to regulate Golgi shape and size which is essential for efficient cellular protein handling. The complex formation allows GOLPH3 to dynamically influence Golgi positioning within the cell an activity important for many cellular processes.

Pathways

The protein GOLPH3 interacts in the mTOR signaling and glycosylation pathways. The protein is associated with the regulation of mTOR signaling through its interaction with proteins like PIK3CA and AKT1. Additionally its role in glycosylation supports protein maturation and trafficking. These pathways are fundamental in cellular growth proliferation and survival and GOLPH3's involvement highlights its multifunctional nature in cellular homeostasis.

GOLPH3 has connections to cancer and Alzheimer's disease. Its overexpression is linked to tumor development and progression in some cancers as it influences cell proliferation and survival through the mTOR pathway. In Alzheimer's disease abnormal Golgi structure and function have been noted and the dysregulation of GOLPH3 may contribute to the pathology. The association with proteins like tau and amyloid-beta in Alzheimer's further indicates the potential impact of GOLPH3 misregulation on disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.

See full target information GOLPH3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 16:1518573 PubMed39944595

2025

GOLPH3 inhibits glioma cell apoptosis through the JNK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shao Xie,Jiahai Ding,Zhaohao Wang,Hengliang Shi,Zheng-Quan Yu

eLife 13: PubMed38346162

2024

A methylation-phosphorylation switch controls EZH2 stability and hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Guo,Rebecca C Lim,Keshari Rajawasam,Tiffany Trinh,Hong Sun,Hui Zhang

Cancer cell international 22:35 PubMed35073936

2022

Golgi phosphoprotein 3 promotes angiogenesis and sorafenib resistance in hepatocellular carcinoma via upregulating exosomal miR-494-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Gao,Zheng Yin,Yunling Qi,Hong Peng,Wenbin Ma,Ruizhi Wang,Wen Li

European journal of histochemistry : EJH 64: PubMed33131267

2020

miR-126 targeting GOLPH3 inhibits the epithelial-mesenchymal transition of gastric cancer BGC-823 cells and reduces cell invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jiancai Ouyang,Fuhu Song,He Li,Rui Yang,Haicheng Huang

Frontiers in oncology 10:575358 PubMed33134174

2020

GOLPH3 Promotes Cancer Growth by Interacting With STIP1 and Regulating Telomerase Activity in Pancreatic Ductal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kebing Wang,Shuai Jiang,Anpei Huang,Ying Gao,Baogang Peng,Zhi Li,Wenbin Ma,Zhou Songyang,Shihong Zhang,Meifang He,Wen Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GOLPH3/MIDAS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (AB236296)

Immunoprecipitation - Anti-GOLPH3/MIDAS antibody (AB236296)

Western blot - Anti-GOLPH3/MIDAS antibody (AB236296)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise