Anti-GOLPH3/MIDAS antibody

• ICC

AbReview28630****

Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (AB91492)

ab91492 staining GOLPH3/MIDAS in human K562 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab91492 at a 1/150 dilution for 1 hour at 22°C. The secondary used was a DyLight 594 conjugated donkey anti-rabbit polyclonal used at a 1/200 dilution.Blue is nucleus staining by DAPI, red is GOLPH3/MIDAS.

Image courtesy of Armen Petrosyan by Abreview.

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PubMed

Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (AB91492)

ab91492, staining GOLPH3/MIDAS (red) in HCV-infected Huh 7.5.1 cells, by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in 3% paraformaldehyde, permeabilized and blocked in antibody-binding buffer for 2 hours at 4°C. Samples were incubated with primary antibody and a fluorescence-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Image from Bishé B et al., J Biol Chem 287:27637-47 (2012)Fig 6.; doi: 10.1074/jbc.M112.346569; August 10, 2012, The Journal of Biological Chemistry, 287, 27637-27647.

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Supplier Data

Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (AB91492)

Immunofluorescence of GOLPH3/MIDAS in Rat Lung cells using ab91492 at 20 ug/ml.

• WB

Unknown

Western blot - Anti-GOLPH3/MIDAS antibody (AB91492)

Lane 1:

Western blot - Anti-GOLPH3/MIDAS antibody (ab91492) at 0.5 µg/mL

Lane 2:

Western blot - Anti-GOLPH3/MIDAS antibody (ab91492) at 1 µg/mL

All lanes:

rat lung tissue lysate at 15 µg

Predicted band size: 34 kDa

Observed band size: 28 kDa,38 kDa,42 kDa,50 kDa

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• ICC

PubMed

Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (AB91492)

ab91492, staining GOLPH3/MIDAS in bovine aortic endothelial cells, by Immunocytochemistry/ Immunofluorescence.

Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100/PBS for 15 min at RT and blocked with 5% donkey serum/0.3% BSA/PBS for 30 min at RT. Samples were incubated with primary antibody at 1/100 dilution overnight at 4°C. An AlexaFlour®568-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody.

Image from Sangwung P et al., PLoS One. 2012;7(2):e31564. Epub 2012 Feb 21. Fig 3.; doi:10.1371/journal.pone.0031564; February 21, 2012, PLoS ONE 7(2): e31564.