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Rabbit Recombinant Monoclonal GOLPH4/GPP130 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.


Images

Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (AB197595), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH4/GPP130 antibody [EPR13439] (AB197595), expandable thumbnail
  • Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (AB197595), expandable thumbnail
  • Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (AB197595), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/500 - 1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.

Alternative names

GIMPC, GOLPH4, GPP130, GOLIM4, Golgi integral membrane protein 4, Golgi phosphoprotein 4, Golgi-localized phosphoprotein of 130 kDa, GIMPc, Golgi phosphoprotein of 130 kDa

Recommended products

Rabbit Recombinant Monoclonal GOLPH4/GPP130 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13439
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GOLPH4 also known as GPP130 and Golgi integral membrane protein 4 is a protein with a mass of approximately 130 kDa. It plays an important role in the organization and maintenance of the Golgi apparatus structure. GOLPH4 is predominantly found in the cis-Golgi network and it is involved in the cellular sorting and trafficking of proteins and lipids. The protein functions as a tethering factor and it may participate in the transport of cargo from the Golgi to other cellular locations.

Biological function summary

The role of GOLPH4 centers around its involvement in the protein trafficking process within cells. It acts as part of the complex tasked with maintaining Golgi organization which is important for facilitating accurate cargo sorting and delivery. GOLPH4's location and structure enable it to interact with motor proteins and other Golgi matrix proteins aiding in vesicle tethering and fusion processes.

Pathways

GOLPH4 is involved in essential cellular pathways such as the secretion and endocytic trafficking pathways. It interacts with other Golgi-resident proteins like GM130 and giantin which are essential for the movement of materials through the Golgi and to their final destinations. These pathways ensure that proteins and lipids reach their intended areas within the cell or are secreted to the extracellular space which is important for maintaining cellular function and homeostasis.

Associated diseases and disorders

Disturbances in GOLPH4 function have been associated with conditions such as cancer and neurodegenerative diseases. Aberrant expression or mutations in this protein can lead to misregulation of Golgi functions affecting overall cellular trafficking systems. GOLPH4's interaction with proteins involved in these conditions such as p53 in cancer or tau in neurodegenerative diseases highlights its potential as a target for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595), expandable thumbnail

    Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)

    Lanes 1-4: Merged signal (red and green). Green - ab197595 observed at 130 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab197595 Anti-GOLPH4/GPP130 antibody [EPR13439] was shown to specifically react with GOLPH4/GPP130 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line ab266123 (knockout cell lysate Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell lysate ab257455) was used. Wild-type and GOLPH4/GPP130 knockout samples were subjected to SDS-PAGE. ab197595 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: GOLIM4 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line (Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line ab266123)

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: MOLT-4 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 82 kDa

    Observed band size: 130 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)

    Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling GOLPH4/GPP130 with ab197595 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human renal adenocarcinoma tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595), expandable thumbnail

    Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature PMID:20130081 and PMID:9201717

    All lanes: Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595) at 1/2500 dilution

    All lanes: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 82 kDa

    Observed band size: 130 kDa

    Exposure time: 1min

  • Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595), expandable thumbnail

    Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:)20130081 and 9201717

    All lanes: Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595) at 1/5000 dilution

    Lane 1: HepG2(Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

    Lane 2: 293(Human embryonic kidney) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 82 kDa

    Observed band size: 130 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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